Cytotoxic, Anti-Inflammatory and Adipogenic Effects of Inophyllum D, Calanone, Isocordato-oblongic acid, and Morelloflavone on Cell Lines.
10.20307/nps.2016.22.2.122
- Author:
Muhammad TAHER
1
;
Amnani AMINUDDIN
;
Deny SUSANTI
;
Nurul Iman AMINUDIN
;
Shamsul ON
;
Farediah AHMAD
;
Hanisuhana HAMIDON
Author Information
1. Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Pahang, Malaysia. mtaher@iium.edu.my
- Publication Type:Original Article
- Keywords:
Cytotoxic;
Anti-inflammatory;
Adipogenic
- MeSH:
Adenocarcinoma;
Adipocytes;
Breast;
Calophyllum;
Cell Line*;
Coumarins;
Garcinia;
In Vitro Techniques;
Macrophages;
MCF-7 Cells
- From:Natural Product Sciences
2016;22(2):122-128
- CountryRepublic of Korea
- Language:English
-
Abstract:
This paper reports in vitro cytotoxic, anti-inflammatory and adipocyte diffentiation with adipogenic effects of coumarins inophyllum D (1) and calanone (2), and a chromanone carboxylic acid namely isocordato-oblongic acid (3) isolated from Calophyllum symingtonianum as well as a biflavonoid morelloflavon e (4) isolated from Garcinia prainiana on MCF-7 breast adenocarcinoma RAW 264.7 macrophages and 3T3-L1 preadipocytes cells, respectively. The cytotoxicity study on MCF-7 cell was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Meanwhile, the study of anti-inflammatory effects in RAW 264.7 macrophages and adipogenic effects on 3T3-L1 pre-adipocytes were conducted through nitrite determination assay and induction of adipocyte differentiation, respectively. In the cytotoxicity study, inophyllum D (1) was the only compounds that exhibited significant cytotoxic effect against MCF-7 cell with IC₅₀ of 84 µg/mL. Further, all by inhibiting the compounds have shown anti-inflammatory effects in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages of nitrite concentration with production. In addition, the compounds also exhibited adipogenic effects on 3T3-L1 pre-adipocytes by stimulating lipid formation. Thus, this study may provide significant input in discovery of the potential effects cytotoxic, anti-inflammatory and adipogenic agents.
