Low dietary inorganic phosphate affects the lung growth of developing mice.
10.4142/jvs.2009.10.2.105
- Author:
Cheng Xiong XU
1
;
Hua JIN
;
Youn Sun CHUNG
;
Ji Young SHIN
;
Soon Kyung HWANG
;
Jung Taek KWON
;
Sung Jin PARK
;
Eun Sun LEE
;
Arash MINAI-TEHRANI
;
Seung Hee CHANG
;
Min Ah WOO
;
Mi Suk NOH
;
Gil Hwan AN
;
Kee Ho LEE
;
Myung Haing CHO
Author Information
1. Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. mchotox@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Akt;
fibroblast growth factor;
inorganic phosphate;
lung
- MeSH:
Animals;
Blotting, Western;
Carrier Proteins/metabolism;
Immunohistochemistry;
Lung/drug effects/enzymology/*growth & development/metabolism;
Male;
Mice;
Mice, Transgenic;
Phosphoproteins/metabolism;
Phosphorus, Dietary/*administration & dosage;
Phosphorylation;
Phosphotransferases (Alcohol Group Acceptor)/metabolism;
Proto-Oncogene Proteins c-akt/metabolism;
Sodium-Phosphate Cotransporter Proteins, Type IIa/*metabolism
- From:Journal of Veterinary Science
2009;10(2):105-113
- CountryRepublic of Korea
- Language:English
-
Abstract:
Inorganic phosphate (Pi) plays a critical role in diverse cellular functions, and regulating the Pi balance is accomplished by sodium-dependent Pi co-transporter (NPT). Pulmonary NPT has recently been identified in mammalian lungs. However, to date, many of the studies that have involved Pi have mainly focused on its effect on bone and kidney. Therefore, current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla/firefly luciferase dual reporter gene. Two-weeks old male mice divided into 2 groups and these groups were fed either a low PI diet or a normal control diet (normal: 0.5% Pi, low: 0.1% Pi) for 4 weeks. After 4 weeks of the diet, all the mice were sacrificed. Their lungs were harvested and analyzed by performing luciferase assay, Western blotting, kinase assay and immunohistochemistry. Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2. These results suggest that optimally regulating Pi consumption may be important to maintain health.
