Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms.
10.4142/jvs.2009.10.2.121
- Author:
Hye Kwon KIM
1
;
Jeong Sun YANG
;
Hyoung Joon MOON
;
Seong Jun PARK
;
Yuzi LUO
;
Chul Seung LEE
;
Dae Sub SONG
;
Bo Kyu KANG
;
Soo Kyung ANN
;
Chan Hyuk JUN
;
Bong Kyun PARK
Author Information
1. Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea. parkx026@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
ORF5;
phylogenetic;
PRRSV;
type;
viremia
- MeSH:
Animals;
Epitopes, B-Lymphocyte/immunology;
Epitopes, T-Lymphocyte/immunology;
Evolution, Molecular;
Korea;
*Open Reading Frames;
Phylogeny;
Pilot Projects;
Porcine Reproductive and Respiratory Syndrome/blood/genetics/immunology/*virology;
Porcine respiratory and reproductive syndrome virus/*genetics/immunology;
RNA, Viral/chemistry/genetics;
Reverse Transcriptase Polymerase Chain Reaction/veterinary;
Swine;
Viral Vaccines/immunology/standards;
Viremia/genetics/immunology/virology
- From:Journal of Veterinary Science
2009;10(2):121-130
- CountryRepublic of Korea
- Language:English
-
Abstract:
The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic sera collected from 2007 to 2008. And of the type I PRRSVs, one subgroup had 87.2~88.9% similarity with the Lelystad virus, showing a close relationship with the 27~2003 strain of Spain. The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4. Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%). The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4. In conclusion, the existence of type I PRRSV, which was genetically different from Lelystad virus (Prototype of type I PRRSV), and heterologous type II PRRSVs of viremic pigs detected even in the MLV-vaccinating farms indicated the need for new vaccine approaches for the control of PRRSV in Korea.
