Detection Of Antibodies To Ena In The Systemic Lupus Erythematosus By Immunoblotting: A Cornparision Of Irnmunoblotting And Double Immunodiffusion.
- Author:
Sung Hwan PARK
1
;
Sang Heon LEE
;
Chul Soo CHO
;
Ho Youn KIM
Author Information
1. Division of Rheumatology, Department of Internal Medicine Catholic University Medical College, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
systemic lupus erythematosus;
ENA;
Immunoblot
- MeSH:
Antibodies*;
Antigens, Nuclear;
Autoantibodies;
Humans;
Immunoblotting*;
Immunodiffusion*;
Lupus Erythematosus, Systemic*;
Prevalence;
Rheumatic Diseases
- From:The Journal of the Korean Rheumatism Association
1995;2(1):34-41
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Systemic Lupus Erythematosus(SLE) is characterized by various autoantibodies to a variety of nuclear antigens. Certain extractable nuclear antigens(ENA) have been served as extremly useful aids in differentiation of clinical subset, diagnostic marker and in the detection of early forms of systemic rheumatic diseases. This study was to employ immunoblot to determine the prevalences of antibodies to ENA in SLE compared with immunodiffusion. METHODS: Sera were obtained from 127 SLE patients. Antibodies to ENA were assessed by double immunodiffusion (DID) and immunoblot method. RESULTS: Using the immunblot method, the prevalences of antibodies to ENA were as follows: The antibody to Sm was 27%, UIRNP 33.6%, Ro 41.8%, La 14%, ribosomal P 14% and Ku 4%. The prevalences of antibodies to ENA by DID were as follows: The antibody to Sm was 15%, RNP 24.5%, Ro 54.3% and La 9.4%. CONCLUSIONS: Compared with immunodiffusion, results using immunoblot showed greater sensitivity in the detection of autoantibodies to ENA in SLE.
