Development of a Synthetic Surfactant Using a Surfactant Protein-C Peptide Analog: In Vitro Studies of Surface Physical Properties.
10.3349/ymj.2016.57.1.203
- Author:
Chong Woo BAE
1
;
Sung Hoon CHUNG
;
Yong Sung CHOI
Author Information
1. Department of Pediatrics, Kyung Hee University School of Medicine, Seoul, Korea. baecw@khnmc.or.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Pulmonary surfactant;
surfactant protein-C;
synthetic peptide syntheses;
respiratory distress syndrome;
surface tension
- MeSH:
1,2-Dipalmitoylphosphatidylcholine/analogs & derivatives;
Adsorption;
Amino Acid Sequence/*genetics;
Animals;
C-Peptide/*chemistry;
Cattle;
Humans;
Infant, Newborn;
Pulmonary Surfactant-Associated Protein C/*chemical synthesis/pharmacology;
Pulmonary Surfactants/*chemical synthesis/pharmacology;
Respiratory Distress Syndrome, Newborn/*drug therapy;
*Surface Properties;
*Surface Tension;
Surface-Active Agents
- From:Yonsei Medical Journal
2016;57(1):203-208
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Pulmonary surfactant (PS) replacement has been the gold standard therapy for neonatal respiratory distress syndrome; however, almost all commercial PSs contain animal proteins. We prepared a synthetic PS by using a human surfactant protein (SP) analog and evaluated its in vitro properties. MATERIALS AND METHODS: A peptide sequence (CPVHLKRLLLLLLLLLLLLLLLL) of human SP-C was chosen to develop the peptide analog (SPa-C). The new synthetic SP-C PS (sSP-C PS) was synthesized from SPa-C, dipalmitoyl phosphatidylcholine, phosphatidyl glycerol, and palmitic acid. Physical properties of the sSP-C PS were evaluated by measuring the maximum and minimum surface tensions (STs), surfactant spreading, and adsorption rate. In addition, we recorded an ST-area diagram. The data obtained on sSP-C PS were subsequently compared with those of purified natural bovine surfactant (PNBS), and the commercial product, Surfacten(R). RESULTS: The sSP-C PS and Surfacten(R) were found to have maximum ST values of 32-33 mN/m, whereas that of PNBS was much lower at 19 mN/m. The minimum ST values of all three products were less than 10 mN/m. The values that were measured for the equilibrium ST of rapidly spreading sSP-C PS, Surfacten(R), and PNBS were 27, 27, and 24 mN/m, respectively. The surface adsorptions were found to be the same for all three PSs (20 mN/m). ST-area diagrams of sSP-C PS and Surfacten(R) revealed similar properties. CONCLUSION: In an in vitro experiment, the physical properties exhibited by sSP-C PS were similar to those of Surfacten(R). Further study is required to evaluate the in vivo efficacy.
