Arg72Pro Polymorphism and Exon 7 Codon 249 Mutation of Plasma DNA p53 Gene in Early Hepatocellular Carcinoma Patients with Hepatitis B Virus Infection in a Korean population.
- Author:
Yongjung PARK
1
;
Jong Han LEE
;
Eun Young LEE
;
Hyon Suk KIM
Author Information
1. Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea. kimhs54@yuhs.ac
- Publication Type:Original Article
- Keywords:
Hepatocellular carcinoma;
p53;
Hepatitis B virus;
Polymorphism
- MeSH:
Biomarkers;
Carcinoma, Hepatocellular;
Codon;
DNA;
Exons;
Genes, p53;
Genotype;
Hepatitis;
Hepatitis B;
Hepatitis B virus;
Homozygote;
Humans;
Plasma;
Polymorphism, Restriction Fragment Length;
Prevalence;
Protein Precursors;
Prothrombin;
Risk Factors
- From:Journal of Laboratory Medicine and Quality Assurance
2010;32(2):255-262
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: This study was performed to investigate on the genotypic frequencies of p53 Arg72Pro polymorphism and the prevalence of p53 codon 249 mutation in hepatocellular carcinoma patients. METHODS: Plasma DNAs were extracted from the samples of 44 early HCC cases, 24 chronic B-viral hepatitis patients and 27 healthy individuals. Serum levels of AFP, PIVKA-II, and HBV DNA-positive rates among the study groups were also compared. PCR-based restriction fragment length polymorphism method was used to determine p53 Arg72Pro genotype and to detect codon 249 mutation. RESULTS: Serum AFP and PIVKA-II level, Edmondson grade, tumor size and frequency of HBV DNA-positivity among HCC group according to Arg72Pro genotypes showed no statistically significant difference. The frequencies of Arg72Pro genotypes (Arg/Arg, Arg/Pro, Pro/Pro) were respectively as follows: 34.1%, 47.7%, 18.2% in HCC group; 29.2%, 54.2%, 16.7% in hepatitis group; 29.6%, 55.6%, 14.8% in control group. Pro homozygote genotype had a higher risk for developing HCC by adjusted OR (1.529, 95% CI 0.325-7.193), but not statistically significant (P=0.591). No codon 249 mutation was found among 44 HCC cases. CONCLUSIONS: Pro homozygote was around 16% in all study groups, and did not statistically increase risks to developing HCC. We suggest that Arg72Pro polymorphism of p53 gene is not a significant risk factor in early hepatocarcinogenesis.
