Effect of Kanamycin on Calcium Current of Rabbit Ventricular Myocyte.
10.4070/kcj.1995.25.2.520
- Author:
Duck Sun AHN
;
Kook In PARK
;
Bok Soon KANG
- Publication Type:Original Article
- Keywords:
Kanmamycin;
Rabbit ventricular cell;
Ca2+ current;
Ca2+ antagonist
- MeSH:
Action Potentials;
Animals;
Calcium*;
Communicable Diseases;
Depression;
Guinea Pigs;
Kanamycin*;
Muscle Cells*;
Myocardium;
Neomycin;
Verapamil
- From:Korean Circulation Journal
1995;25(2):520-530
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
It has been reported that kanamycin, a useful agent in the treatment of gram-negative and other infectious disease, has a negative inotropic action in isolated cardiac muscle preparation and also it decreases the amplitude of high K+ -induced action potential of guinea pig atrium. These findings imply that kanamycin has a property of Ca2+ antagonist. In this study, the effects of kanamycin on cardiac Ca2+ current were investigated in isolated rabbit ventricular cells by using a whole cell clamp nethod.The results are summarized as follows ; 1) Kanamycin caused a depression fo cardiac Ca2+ current in a dose dependent manner and its effect was observed in a whole memberane potential renge. 2) The decreasing effect of kanamycin on Ca2+ current was inhibited by high Ca(2+)-Tyrode solution and such inhibition was also observed in high Sr2+-Tyrode solution, but in the case of Cd2+, well known inorganic Ca2+ antagonist, the current through Ca2+ channel was greatly decreased when the perfuaste was changed from high Ca(2+)-Tyrode to high Sr(2+)-Tyrode solution. 3) In the presense of kanamycin, the decreasing pattern of Ca2+current by repetitve depolarization was not specific as in the case of verapamil. 4) Neomycin decreased Ca2+ current similar to kanamycin.From the above result, it may be concluded that kanamycin has competitive antagonistin effect on cardiac Ca2+ current.
