Lactoferrin Combined with Retinoic Acid Stimulates B1 Cells to Express IgA Isotype and Gut-homing Molecules.
- Author:
Seong Ho KANG
1
;
Bo Ra JIN
;
Hyeon Jin KIM
;
Goo Young SEO
;
Young Saeng JANG
;
Sun Jin KIM
;
Sun Jin AN
;
Seok Rae PARK
;
Woan Sub KIM
;
Pyeung Hyeun KIM
Author Information
- Publication Type:Original Article
- Keywords: Lactoferrin; Retinoic acid; Peritoneal B1 cell; IgA; Gut-homing molecule
- MeSH: Animals; B-Lymphocytes; Immunoglobulin A*; Immunoglobulin Class Switching; Immunoglobulin G; Lactoferrin*; Mice; Spleen; Transforming Growth Factor beta1; Tretinoin*
- From:Immune Network 2015;15(1):37-43
- CountryRepublic of Korea
- Language:English
- Abstract: It is well established that TGF-beta1 and retinoic acid (RA) cause IgA isotype switching in mice. We recently found that lactoferrin (LF) also has an activity of IgA isotype switching in spleen B cells. The present study explored the effect of LF on the Ig production by mouse peritoneal B cells. LF, like TGF-beta1, substantially increased IgA production in peritoneal B1 cells but little in peritoneal B2 cells. In contrast, LF increased IgG2b production in peritoneal B2 cells much more strongly than in peritoneal B1 cells. LF in combination with RA further enhanced the IgA production and, interestingly, this enhancement was restricted to IgA isotype and B1 cells. Similarly, the combination of the two molecules also led to expression of gut homing molecules alpha4beta7 and CCR9 on peritoneal B1 cells, but not on peritoneal B2 cells. Thus, these results indicate that LF and RA can contribute to gut IgA response through stimulating IgA isotype switching and expression of gut-homing molecules in peritoneal B1 cells.
