Role of Cytosolic Calcium Ion and Expression of Proliferation Responsive Gene of Cultured Medial Smooth Muscle Cells Obtained from rat Aortas with Atherosclerotic Changes Induced by High Cholesterol Feeding.
- Author:
Hyung Joon YOO
- Publication Type:Original Article
- Keywords:
Diabetes;
Atherosclerosis;
Cytosolic calcium;
Calcium channel gene
- MeSH:
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester;
Animals;
Aorta*;
Atherosclerosis;
Calcium Channels;
Calcium*;
Cell Count;
Cholesterol*;
Collagen;
Cytosol*;
Diet;
Fura-2;
Muscle, Smooth*;
Muscle, Smooth, Vascular;
Myocytes, Smooth Muscle*;
Rats*;
Verapamil
- From:Journal of the Korean Geriatrics Society
2000;4(4):251-256
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Vascular smooth muscle cells (VSMCs) proliferation is an important process in the pathogenesis of atherosclerosis. VSMCs proliferation is closedly related to the cytosolic calcium flux via L-type voltage dependent calcium channel. OBJECTIVES: To investigate the role of cytosolic Ca(2+) in the proliferation of VSMCs. METHODS: The proliferation of aortic vascular smooth muscle cells of rats(1% cholesterol diet fed rats and general diet fed ones), acquired by enzymatic method. Cell counting, and calcium agonists(Bay K 8644 10(-6)M) or calcium antagonists(verapamil 10(-6)M). Cytosolic calcium ion was measured with Fura 2 method. Calcium channel gene expresssion was detected with RT-PCR method. RESULTS: Masson Trichrome staining shows more disturbed cell lining, more VSMCs, more degenerated media, more collagen laydown, and more adventitial fat in the aorta of cholesterol-fed rats than in that of general diet-fed ones. VSMCs of cholesterol fed-rats were moreproliferated than those of general diet fed-ones. Bay K 8644 enhanced VSMCs proliferation of both groups. Verapamil blocked the incremental effects induced by Bay K 8644. Expression of calcium channel gene was enhanced by Bay K 8644 and was reversed by verapamil. CONCLUSION: The enhanced proliferation of atherosclerotic VSMCs is associated with the increment in cytosolic calcium, which is accomplshed through the expression of L-type voltage dependent calcium channel.
