Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear.
10.3347/kjp.2006.44.4.313
- Author:
Sun Joo LEE
1
;
Hae Jin JEONG
;
Ji Eun LEE
;
Jong Soo LEE
;
Ying Hua XUAN
;
Hyun Hee KONG
;
Dong Il CHUNG
;
Mee Sun OCK
;
Hak Sun YU
Author Information
1. Department of Parasitology, School of Medicine, Pusan National University, Busan 602-739, Korea. hsyu@pusan.ac.kr
- Publication Type:Case Reports ; Research Support, Non-U.S. Gov't
- Keywords:
Acanthamoeba castellanii Ma;
orthokeratology;
amoebic keratitis;
polyhexamethylene biguanide (PHMB);
18S rDNA sequence analysis
- MeSH:
*Sequence Analysis, DNA;
RNA, Ribosomal, 18S/genetics;
Polymorphism, Restriction Fragment Length;
Parasitic Sensitivity Tests;
Myopia/therapy;
Itraconazole/administration & dosage;
Humans;
Female;
Disinfectants/administration & dosage;
DNA, Ribosomal/analysis;
DNA, Protozoan/analysis;
DNA, Mitochondrial/analysis;
Contact Lenses/*adverse effects;
Chlorhexidine/administration & dosage;
Biguanides/administration & dosage;
Astigmatism/therapy;
Antiprotozoal Agents/administration & dosage;
Animals;
Adolescent;
Acanthamoeba Keratitis/drug therapy/*parasitology;
Acanthamoeba/classification/*genetics/*isolation & purification
- From:The Korean Journal of Parasitology
2006;44(4):313-320
- CountryRepublic of Korea
- Language:English
-
Abstract:
In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patient's ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed.
