Hydrogenosomal activity of Trichomonas vaginalis cultivated under different iron conditions.
10.3347/kjp.2006.44.4.373
- Author:
Yong Seok KIM
1
;
Hyun Ouk SONG
;
Ik Hwa CHOI
;
Soon Jung PARK
;
Jae Sook RYU
Author Information
1. Department of Biochemistry and Molecular Biology, Hanyang University College of Medicine, Seoul 133-791, Korea.
- Publication Type:Brief Communication
- Keywords:
Trichomonas vaginalis;
hydrogenosomal enzyme;
gene expression;
hydrogenosomal membrane potential;
iron;
DiOC6;
PFOR;
hydrogenase;
malic enzyme;
ferredoxin
- MeSH:
Trichomonas vaginalis/*growth & development;
Reverse Transcriptase Polymerase Chain Reaction;
Pyruvate Synthase/genetics/metabolism;
Organelles/*enzymology/metabolism/*physiology;
Membrane Potentials;
Malate Dehydrogenase/genetics/metabolism;
Iron/*metabolism;
Hydrogenase/genetics/metabolism;
Hydrogen/*metabolism;
Humans;
Gene Expression Regulation, Enzymologic;
*Gene Expression Regulation;
Ferredoxins/genetics/metabolism;
Culture Media;
Animals
- From:The Korean Journal of Parasitology
2006;44(4):373-378
- CountryRepublic of Korea
- Language:English
-
Abstract:
To evaluate whether iron concentration in TYM medium influence on hydrogenosomal enzyme gene expression and hydrogenosomal membrane potential of Trichomonas vaginalis, trophozoites were cultivated in irondepleted, normal and iron-supplemented TYM media. The mRNA of hydrogenosomal enzymes, such as pyruvate ferredoxin oxidoreductase (PFOR), hydrogenase, ferredoxin and malic enzyme, was increased with iron concentrations in T. vaginalis culture media, measured by RT-PCR. Hydrogenosomal membrane potentials measured with DiOC6 also showed similar tendency, e.g. T. vaginalis cultivated in iron-depleted and iron-supplemented media for 3 days showed a significantly reduced and enhanced hydrogenosomal membrane potential compared with that of normal TYM media, respectively. Therefore, it is suggested that iron may regulate hydrogenosomal activity through hydrogenosomal enzyme expression and hydrogenosomal membrane potential.
