Benzoapyrene-Induced DNA-Protein Crosslinks in Cultured Human Lymphocytes and the Role of the GSTM1 and GSTT1 Genotypes.
10.3346/jkms.2002.17.3.316
- Author:
Hye Sook PARK
1
;
Eun Hee HA
;
Kwan Hee LEE
;
Yun Chul HONG
Author Information
1. Department of Preventive Medicine, College of Medicine, Ewha Woman's University, Seoul, Korea. ychong@inah.ac.kr
- Publication Type:Original Article ; In Vitro
- Keywords:
Polymorphism;
Genetics;
Glutathione Transferase;
Benzopyrenes;
DNA-Binding Proteins
- MeSH:
Adult;
Benzo(a)pyrene/*toxicity;
Cells, Cultured;
Cross-Linking Reagents/*toxicity;
DNA-Binding Proteins;
Dose-Response Relationship, Drug;
Genotype;
Glutathione Transferase/*genetics;
Humans;
Lymphocytes/cytology/drug effects/*physiology;
Male;
Polymorphism, Genetic
- From:Journal of Korean Medical Science
2002;17(3):316-321
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated the influence of glutathione S-transferase M1 (GSTM1) and glutathione S-transferase T1 (GSTT1) polymorphisms upon DNA-protein crosslinks (DPC) induced by benzo[a]pyrene (B[a]P) in cultured human lymphocytes. Lymphocyte samples were collected from 30 healthy nonsmoking hospital administrative workers. DPC was detected with KCl-SDS assay and the distributions of GSTM1 and GSTT1 were determined by polymerase chain reaction. B[a]P was found to induce a significant dose-responsive increase in cytotoxicity and DPC regardless of the genotypes (p<0.05). We did not find statistically significant genetic modification effect of GSTM1 and GSTT1 polymorphisms in the cytotoxicity and DPC formation (p>0.05). In terms of the genes examined, the level of cytotoxicity and DPC formation were found to be highest in the GSTM1-null and GSTT1-null cells. In conclusion, B[a]P induced a significant increase in the cytotoxicity and the level of DPC formation in cultured human lymphocytes. Our findings suggest that DPC could be used as a biomarker of B[a]P exposure.
