The Simplifed Hair Baiting Technique and its Practical Application.
- Author:
Byung Jin LEE
;
Soon Bong SUH
- Publication Type:Original Article
- MeSH:
Animals;
Anti-Bacterial Agents;
Dihydroergotamine;
Hair*;
Horses;
Penicillins;
Soil;
Streptomycin
- From:Korean Journal of Dermatology
1982;20(5):695-702
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In order to avoid contamination attributed to Vanbreuseghem hair haiting technique in the laboratory, the author contrived the following modified method. After adding 100ml of antibiotics solution (mixture of 500mg of Cyclohexamide, 20, 000u of Penicillin and 40mg of Streptomycin in 1,000ml of distilled water) in PVC phial containing 100gm of soil, it was gently agitated for an hour and lefted to be settled for 12 hours. The suspended solution obtained and a batch of horse hair were randomly distributed on a Petri dishfitted with blue cloths, then cultured at 24C'. The overall results obtained from this modified method were not as satisfying as that of the Vanbreuseghem method but a few advantages such as simplicity of the procedure and significantly lower laboratory contamination implicated its practicality. Furthermore, this method was also found to be useful in the first isolation of infected animal hairs, hair perforation tests and as a substitution for artificial media. For the cross mating, a patch of blue cloths was laid on top cf moist sterilized soil evenly spreaded on a Petri dish. Then hair-baiting procedure was performed and good results were obtained.
