Metacercarial proteins interacting with WD40-repeat protein of Clonorchis sinensis.
10.3347/kjp.2007.45.3.229
- Author:
Pyo Yun CHO
1
;
Tae Im KIM
;
Shunyu LI
;
Sung Jong HONG
;
Min Ho CHOI
;
Sung Tae HONG
;
Yong Je CHUNG
Author Information
1. Department of Parasitology, Chung-Ang University College of Medicine, Seoul, Korea. hongsj@cau.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Clonorchis sinensis;
WD40-repeat protein;
actin
- MeSH:
Animals;
Antibodies, Helminth/metabolism;
Clonorchis sinensis/*physiology;
Electrophoresis, Gel, Two-Dimensional/veterinary;
Helminth Proteins/chemistry/*isolation & purification/metabolism;
Hydrogen-Ion Concentration;
Immunoglobulin G/chemistry;
Microfilament Proteins/chemistry/*isolation & purification/metabolism
- From:The Korean Journal of Parasitology
2007;45(3):229-232
- CountryRepublic of Korea
- Language:English
-
Abstract:
The WD40-repeat proteins serve as a platform coordinating partner proteins and are involved in a range of regulatory cellular functions. A WD40-repeat protein (CsWD1) of Clonorchis sinensis previously cloned is expressed stage-specifically in the tegumental syncytium of C. sinensis metacercariae. In the present study, interacting proteins with the CsWD1 protein was purified by immunoprecipitation and 2 dimension gel electrophoresis from the C. sinensis metacercaria soluble extract, and tryptic peptides were analyzed by LC/ESI-MS. Putative partner proteins were annotated to be actin-2, glyceraldehyde-3-phosphate dehydrogenase, and hypothetical and unmanned proteins. The CsWD1 protein was predicted to contain 3 conserved actin-interacting residues on its functional surface. With these results, the CsWD1 protein is suggested to be an actin-interacting protein of C. sinensis.
