Effects of occupation, life style and genetic polymorphism of CYP1A1, GSTM1, and GSTT1 on urinary 1-hydroxypyrene and 2-naphthol concentration.
- Author:
Heon KIM
1
;
Hyun Sul LIM
;
Jong Won KANG
;
Holik LEE
;
Yong Dae KIM
;
Hong Mei NAN
;
Chul Ho LEE
Author Information
1. Department of Preventive Medicine, College of Medicine, Chungbuk National University, Korea.
- Publication Type:Original Article
- Keywords:
PAH Coke oven worker;
CYP1A1;
GSTM1;
GSTT
- MeSH:
Coke;
Cytochrome P-450 CYP1A1*;
Cytochrome P-450 Enzyme System;
Eating;
Genotype;
Glutathione;
Inhalation Exposure;
Life Style*;
Meat;
Metabolism;
Occupational Exposure;
Occupations*;
Polycyclic Hydrocarbons, Aromatic;
Polymorphism, Genetic*;
Questionnaires;
Smoke;
Smoking
- From:Korean Journal of Occupational and Environmental Medicine
1999;11(4):546-556
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: This study was performed to describe the distribution patterns of urinary 1-hydroxypyrene (1-OHP) and 2naphthol concentration in coke oven workers and workers not occupationally exposed to polycyclic aromatic hydrocarbons (PAH), and to determine the effects of occupation life style, and genetic polymorphism of cytochrome P450 1A1 (CYP1A1), glutathione Stransferase mu 1 (GSTM1) and theta 1 (GSTT1) on urinary 1- OHP and 2-naphthol concentration. METHODS: The study subjects were 19 coke oven workers and 156 shipyard workers. A questionnaire was used to obtain data about detailed smoking and food intake history. Urinary 1OHP and 2naphthol concentration and genetic polymorphism of CYPIA1, GSTM1, and GSTT1 were analyzed. RESULTS: The urinary 1-OHP and 2-naphthol concentration was higher in the coke oven workers and in smokers. Urinary 1-OHP concentration was significantly correlated with time after last intake of roasted meat in non-smoking coke oven workers, whereas urinary 2-naphthol concentration was with amount of cigarette smoking at the sampling day in smoking shipyard workers. Urinary 1-OHP, but not 2-naphthol, concentration of the shipyard workers with Ile/Ile type of CYP1A1 vas significantly lower than that of the shipyard workers with other CYP1A1 genotype. CONCLUSIONS: Urinary 1-OHP would be a better marker for occupational exposure to PAH in coke oven workers, and urinary 2naphthol might be better for non-occupational inhalation exposure to PAH. CYP1A1 would not play an important role in the metabolism of naphthalene but in the metabolism of pyrene.
