Transferable Trimethoprim Resistance in Urine Isolates of Escherichia coli.
- Author:
Sung Yong SEOL
;
Dong Taek CHO
;
Yoo Chul LEE
;
Jung Min KIM
;
Haeng Seop SHIN
;
Hee Kyung CHANG
;
Hyang Soo HYUN
- Publication Type:Original Article
- MeSH:
Aminoglycosides;
Carbenicillin;
Cephalosporins;
Deoxyribonuclease EcoRI;
DNA;
Drug Resistance, Multiple;
Escherichia coli*;
Escherichia*;
Microbial Sensitivity Tests;
Molecular Weight;
Penicillins;
Plasmids;
R Factors;
Sulfisoxazole;
Trimethoprim Resistance*;
Trimethoprim*;
Urinary Tract Infections
- From:Journal of the Korean Society for Microbiology
1997;32(5):553-568
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
One hundred and twenty-two strains of E. coli isolated from urinary tract infection were examined for antibiogram, transferability of trimethoprim (Tp) resistance, incompatibility with F group plasmid and southem hybridization with DHFR I, II, and III probe of Tp-resistant R plasmids. 1. Among 172 Gram negative bacilli isolated from urinary tract infection, 122 (70.9%) were E. coli and 75 strains of them were resistant to trimethoprim (Tp). Most of Tp-resistant isolates were also resistant to penicillins (ampicillin, carbenicillin, and ticarcillin), aminoglycosides (kanamycin and gentamicin), and sulfisoxazole but almost all strains were susceptible to cephalosporins. 2. Most of Tp-resistant strains and E. coli transconjugant derived from them showed multiple drug resistance and various antimicrobial resistance patterns. 3. Thirty-three Tp-resistant strains (45.2%) transferred 35 Tp-resistant plasmids to E. coli recipients but among them 6 transconjugants did not show retransfer of resistance and plasmid DNA were not detected in 2 transconjugants after resistance transfer. 4. Tp-resistant R plasmids ranged from 157 to 67 kb and 8 R plasmids were classified to incompatibilty group IncFI or IncFII ranging from 120 to 83 kb. Three and two R plasmids belonged to IncFII showed similar molecular weight, resistance pattern, and reaction site by southern hybridization with DHFR I probe. Twenty-five plasmids specifically responded on various EcoRI endonuclease fragments to DHFR I probe but not to DHFR II or DHFR III probe. These findings suggest that most of Tp- resistant R plasmids from urine isolates of E. coli were derived from various sources but some plasmids including IncFII R plasmids were probably originated from same or similar sources.
