Usefulness of Disposable Horizontal Electrophoresis.
- Author:
Kyung Hee KIM
;
Jong Suk OH
;
Hyun Chul LEE
;
Jin CHUNG
;
Shi Eun LEE
- Publication Type:Original Article
- MeSH:
Coloring Agents;
Electrodes;
Electrophoresis*;
Running;
Sepharose
- From:Journal of the Korean Society for Microbiology
1997;32(5):577-581
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We designed a new electrophoresis apparatus. This was consisted of the body into which agarose could be poured directly and the cover from which the platinium electrodes were suspended. After running the nucleic acid in this electrophoresis, we observed the band of nucleic acid on the ultraviolet transilluminator without touching the agarose gel, keeping the cover with the electrodes and discarding the gel in body. We compared the variuos types of the electrophoresis apparatus. In the electrophoresis apparatus which body was consisted of the flat bottom, the migration of dyes was the fastest among studied types. The high migration velocity of dyes was due to high electric current.