Polymorphism Analysis of Hepatitis G Virus ( HGV ) Based on RT-PCR and RFLP.
- Author:
Young Suk PARK
;
Eun Mi JOO
- Publication Type:Original Article
- MeSH:
Blood Transfusion;
Drug Users;
Flaviviridae;
GB virus C*;
Genotype;
Hemophilia A;
Hepatitis*;
Hepatitis, Chronic;
Humans;
Korea;
Liver Diseases;
Plasma;
Polymorphism, Restriction Fragment Length*;
RNA;
RNA Viruses;
Sexual Partners
- From:Journal of the Korean Society for Microbiology
1997;32(5):593-600
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Hepatitis G virus (HGV) is a single-strand RNA virus in the Flaviviridae family, it was recently identified from the plasma of a patient with chronic hepatitis. HOV infection may cause acute and chronic liver disease by blood transfusion, drug addicts, hemophilia, and multiple sexual partners. But clinical significance of infectious pathway is still unclear. In this report, we amplified HGV RNA by reverse transcription-PCR (RT-PCR) by primers within the highly conserved 5'-noncoding region (NCR) and used restriction fragment length polymorphism (RFLP) method for the polymorphism analysis of amplified HGV gene. HGV was shown to be present in 7 of 78 (9.0%) from HCV RT-PCR positive serum samples and 5 of 58 (8.6%) from HCV RT-PCR negative serum samples. From the RFLP method HGV divided into four genotypes in 12 positive samples. Therefore, HGV genotype was distributed at least four different types in Korea.
