Stimulatory effects of Bordetella bronchiseptica antigen on bone marrow cells and immune memory responses.
10.14405/kjvr.2014.54.4.203
- Author:
Seol Hwa YIM
1
;
Hong Gu JOO
Author Information
1. Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea. jooh@jejunu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
antigen;
Bordetella bronchiseptica;
immune memory responses;
stimulatory effects;
vaccine
- MeSH:
B-Lymphocytes;
Bone Marrow;
Bone Marrow Cells*;
Bordetella;
Bordetella bronchiseptica*;
Interleukin-6;
Memory*;
Nitric Oxide;
Spleen;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Veterinary Research
2014;54(4):203-208
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Bone marrow is a hematological and immunological organ that provides multiple immune cells, including B lymphocytes, and thus plays a critical role in the efficacy of vaccine. We previously demonstrated that Bordetella (B.) bronchiseptica antigen has high immunogenicity in spleen cells, a peripheral immune organ. In this study, we investigated the immunogenicity of B. bronchiseptica antigen in bone marrow cells, a central immune organ. B. bronchiseptica antigen increased the cellular activity of bone marrow cells and significantly enhanced the production of nitric oxide, IL-6, and TNF-alpha. Bone marrow cells primed with B. bronchiseptica antigen in vivo were harvested and stimulated with the same antigen in vitro. The stimulation of B. bronchiseptica antigen significantly increased the cellular activity and proliferation rate of the primed cells. B. bronchiseptica antigen also greatly induced the production of antigen-specific antibody in the primed cells. Taken together, the present study demonstrated that B. bronchiseptica antigen can stimulate bone marrow cells, a central immune organ, and recall the immune response of the primed bone marrow cells.
