Survival of hypoxic human mesenchymal stem cells is enhanced by a positive feedback loop involving miR-210 and hypoxia-inducible factor 1.
- Author:
Woochul CHANG
1
;
Chang Youn LEE
;
Jun Hee PARK
;
Moon Seo PARK
;
Lee So MAENG
;
Chee Soon YOON
;
Min Young LEE
;
Ki Chul HWANG
;
Yong An CHUNG
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: cell survival; human mesenchymal stem cells; hypoxia; hypoxia inducible factor-1; microRNA-210
- MeSH: Cell Survival; Cobalt; Gene Expression Regulation/*physiology; Humans; Hypoxia-Inducible Factor 1, alpha Subunit/genetics/*metabolism; Mesenchymal Stromal Cells/drug effects/metabolism/*physiology; MicroRNAs/*metabolism; Oxygen/pharmacology; *Oxygen Consumption; RNA, Small Interfering/metabolism
- From:Journal of Veterinary Science 2013;14(1):69-76
- CountryRepublic of Korea
- Language:English
- Abstract: The use of mesenchymal stem cells (MSCs) has emerged as a potential new treatment for myocardial infarction. However, the poor viability of MSCs after transplantation critically limits the efficacy of this new strategy. The expression of microRNA-210 (miR-210) is induced by hypoxia and is important for cell survival under hypoxic conditions. Hypoxia increases the levels of hypoxia inducible factor-1 (HIF-1) protein and miR-210 in human MSCs (hMSCs). miR-210 positively regulates HIF-1alpha activity. Furthermore, miR-210 expression is also induced by hypoxia through the regulation of HIF-1alpha. To investigate the effect of miR-210 on hMSC survival under hypoxic conditions, survival rates along with signaling related to cell survival were evaluated in hMSCs over-expressing miR-210 or ones that lacked HIF-1alpha expression. Elevated miR-210 expression increased survival rates along with Akt and ERK activity in hMSCs with hypoxia. These data demonstrated that a positive feedback loop involving miR-210 and HIF-1alpha was important for MSC survival under hypoxic conditions.
