Serotype- and serogroup-specific detection of African horsesickness virus using phage displayed chicken scFvs for indirect double antibody sandwich ELISAs.
- Author:
Wouter VAN WYNGAARDT
1
;
Cordelia MASHAU
;
Isabel WRIGHT
;
Jeanni FEHRSEN
Author Information
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords: African horsesickness virus; double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA); phage-displayed scFv; serotype-specific
- MeSH: African horse sickness virus/*isolation & purification; Animals; Antibodies, Immobilized; Antibodies, Viral/*immunology; Cercopithecus aethiops; Chickens; Enzyme-Linked Immunosorbent Assay/methods/*veterinary; Immunoglobulin G; *Peptide Library; Serologic Tests/methods/veterinary; Serotyping; Single-Chain Antibodies/*immunology; Vero Cells
- From:Journal of Veterinary Science 2013;14(1):95-98
- CountryRepublic of Korea
- Language:English
- Abstract: There is an ongoing need for standardized, easily renewable immunoreagents for detecting African horsesickness virus (AHSV). Two phage displayed single-chain variable fragment (scFv) antibodies, selected from a semi-synthetic chicken antibody library, were used to develop double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) to detect AHSV. In the DAS-ELISAs, the scFv previously selected with directly immobilized AHSV-3 functioned as a serotype-specific reagent that recognized only AHSV-3. In contrast, the one selected with AHSV-8 captured by IgG against AHSV-3 recognized all nine AHSV serotypes but not the Bryanston strain of equine encephalosis virus. Serving as evidence for its serogroup-specificity. These two scFvs can help to rapidly confirm the presence of AHSV while additional serotype-specific scFvs may simplify AHSV serotyping.
