The association of follicular fluid volume with human oolemma stretchability during intracytoplasmic sperm injection.
10.5653/cerm.2017.44.3.126
- Author:
Taketo INOUE
1
;
Yoshiki YAMASHITA
;
Yoshiko TSUJIMOTO
;
Shuji YAMAMOTO
;
Sayumi TAGUCHI
;
Kayoko HIRAO
;
Mikiko UEMURA
;
Kayoe IKAWA
;
Kazunori MIYAZAKI
Author Information
1. Umeda Fertility Clinic, Osaka, Japan. taketoein@yahoo.co.jp
- Publication Type:Original Article
- Keywords:
Fertilization;
Gonadotropin-releasing hormone;
Infertility;
Membranes;
Oocytes;
Ovarian follicle;
Ovulation induction;
Sperm injections, Intracytoplasmic
- MeSH:
Blastocyst;
Clothing;
Female;
Fertilization;
Follicular Fluid*;
Gonadotropin-Releasing Hormone;
Humans*;
Infertility;
Membranes;
Metaphase;
Oocyte Retrieval;
Oocytes;
Ovarian Follicle;
Ovulation Induction;
Retrospective Studies;
Risk Factors;
Sperm Injections, Intracytoplasmic*
- From:Clinical and Experimental Reproductive Medicine
2017;44(3):126-131
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: Oocyte degeneration often occurs after intracytoplasmic sperm injection (ICSI), and the risk factor is low-quality oocytes. The follicular fluid (FF) provides a crucial microenvironment for oocyte development. We investigated the relationships between the FF volume aspirated from individual follicles and oocyte retrieval, oocyte maturity, oolemma stretchability, fertilization, and development. METHODS: This retrospective study included data obtained from 229 ICSI cycles. Ovarian stimulation was performed according to a gonadotropin-releasing hormone antagonist protocol. Each follicle was individually aspirated and divided into six groups according to FF volume (<1.0, 1.0 to <2.0, 2.0 to <3.0, 3.0 to <4.0, 4.0 to <5.0, and ≥5.0 mL). Oolemma stretchability during ICSI was evaluated using a mechanical stimulus for oolemma penetration, that is, the stretchability was assessed by oolemma penetration with aspiration (high stretchability) or without aspiration (low stretchability). RESULTS: Oocyte retrieval rates were significantly lower in the <1.0 mL group than in the ≥1.0 mL groups (46.0% [86/187] vs. 67.5%–74.3% [172/255 to 124/167], respectively; p<0.01). Low oolemma stretchability was significantly more common in the <1.0 mL group than in the ≥1.0 mL groups during ICSI (22.0% [13/59] vs. 5.8%–9.4% [6/104 to 13/139], respectively; p=0.018). There was a relationship between FF volume and oolemma stretchability. However, there were no significant differences in the rates of fertilization, cleavage, ≥7 cells at day 3, and blastocyst development among all groups. CONCLUSION: FF volume is potentially associated with the stretchability of metaphase II oolemma during ICSI. Regarding oolemma stretchability, ensuring a uniform follicular size during ovarian stimulation is crucial to obtain good-quality oocytes.
