Effect of Etomidate on N-methyl-D-aspartate Induced Neurotoxicity in Rat Cortical Neurons.
10.4097/kjae.2005.49.6.842
- Author:
Yu Mi LEE
1
;
Eun Ho LEE
;
Joung Uk KIM
;
Hyun Jung LEE
;
Byeong Moon HWANG
;
Jin Woo SHIN
;
Seung Woo KU
Author Information
1. Department of Anesthesiology and Pain Medicine, College of Medicine, University of Ulsan, Seoul, Korea. jukim@amc.seoul.kr
- Publication Type:Original Article
- Keywords:
etomidate;
lactate dehydrogenase (LDH);
neurotoxicity;
N-methyl-D-aspartate (NMDA)
- MeSH:
Animals;
Aspartic Acid;
Cell Culture Techniques;
Cell Death;
Culture Media;
Etomidate*;
L-Lactate Dehydrogenase;
N-Methylaspartate*;
Neurons*;
Neuroprotective Agents;
Rats*
- From:Korean Journal of Anesthesiology
2005;49(6):842-846
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The present investigation was undertaken to evaluate the neuroprotective effect of etomidate against N-methyl-D- aspartate (NMDA) induced neurotoxicity in rat cortical neurons by measuring lactate dehydrogenase (LDH). METHODS: The cerebral cortical neurons of fetal rat were grown for 12 days in dissociated cell culture. They were divided into four groups: first group acted as control with no drug administration and other groups treated with either NMDA 100microM or Etomidate (ET) 10microM + NMDA 100microM or ET 100microM + NMDA 100microM After 24 hrs, cell death was assessed by morphology under the light microscope and quantified by measuring the LDH in the culture media. RESULTS: In the light microscopic findings, the intact cortical neurons were increased in the ET groups. NMDA-induced LDH production also significantly suppressed in ET group (P<0.05). There were no significant difference between the ET 10microM and 100microM groups. CONCLUSIONS: These results suggest that the etomidate has protective effect on the cultured rat cortical neurons against NMDA-induced neurotoxicity.
