HLA-B27 Subtypes in Korean Patients with Ankylosing Spondylitis.
10.3343/kjlm.2008.28.1.46
- Author:
Kyung Sun PARK
1
;
So Young KANG
;
Woo In LEE
Author Information
1. Department of Laboratory Medicine, Kyung Hee University College of Medicine, Seoul, Korea. wileemd@khu.ac.kr
- Publication Type:Original Article ; English Abstract
- Keywords:
Ankylosing spondylitis;
HLA-B27 subtypes;
PCR-SSP method
- MeSH:
Adult;
Alleles;
Female;
Gene Frequency;
Genotype;
HLA-B27 Antigen/blood/*genetics;
Humans;
Korea/epidemiology;
Male;
Polymerase Chain Reaction;
Polymorphism, Genetic;
Reagent Kits, Diagnostic;
Spondylitis, Ankylosing/*diagnosis/epidemiology
- From:The Korean Journal of Laboratory Medicine
2008;28(1):46-52
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: HLA-B27 is strongly associated with ankylosing spondylitis (AS), and its subtypes differ in their ethnic distribution. Studies worldwide have shown that B*2701, B*2702, B*2704, B*2705, B*2707, B*2708, B*2714, B*2715, and B*2719 are AS-predisposing subtypes, whereas B*2706 and B*2709 are reported to be negatively associated with AS. The aim of this study was to investigate HLA-B27 polymorphism and clinical features according to subtypes in Korean patients with AS. METHODS: Two hundred thirty samples from patients with impression of AS were analyzed by polymerase chain reaction using a sequence-specific primers (PCR-SSP) method. Pel-Freez SSP Unitray HLA-B*27 kit (Dynal Biotech, USA) including 16 primers was used to define HLA-B27 subtypes from B*2701 to B*2735. RESULTS: Among 230 samples from patients with impression of AS, 171 were HLA-B27 positive, and among 160 patients diagnosed as AS, 154 (96.3%) were HLA-B27 positive, while 17 patients not diagnosed as AS were HLA-B27 positive. Among 154 HLA-B27 positive patients with AS, 142 (92.2%) were typed as B*2705 and 9 (5.8%) were typed as B*2704. Three cases (1.9%) could be interpreted only variously because of their HLA-B27 homogeneous alleles. Between B*2705 and B*2704, no specific HLA-B27 subtype appeared to contribute to AS susceptibility (P=0.60). Difference in clinical features between B*2705 and B*2704 could not be found in this study (P>0.05). CONCLUSIONS: This study verified that HLA-B27 (96.3%) is strongly associated with AS and identified that the major subtypes of HLA-B27 positive patients with AS in Korea are B*2705 (92.2%) and B*2704 (5.8%).
