Quantitation of T-lymphocyte Subsets Using the CELL-DYN Sapphire Automated Haematology Analyser.
- Author:
Jimyung KIM
1
;
Seung RYU
;
Jinsook LIM
;
Gyechul KWON
;
Sunhoe KOO
Author Information
1. Department of Laboratory Medicine, Chungnam National University Hospital, Daejeon, Korea. jmkim@cnuh.co.kr
- Publication Type:Original Article
- Keywords:
CELL-DYN Sapphire;
Flow cytometry;
CD4-positive T-lymphocytes;
CD8-positive T-lymphocytes;
T lymphocyte subsets
- MeSH:
Aluminum Oxide*;
CD4-Positive T-Lymphocytes;
CD8-Positive T-Lymphocytes;
Cell Count;
Flow Cytometry;
Humans;
T-Lymphocyte Subsets*
- From:Journal of Laboratory Medicine and Quality Assurance
2014;36(1):39-47
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Quantitative analysis of T-lymphocyte subsets is used to assess immune competency. Traditionally, T-lymphocyte subset analysis has been performed using flow cytometry, which requires complex instrumentation and relatively skilled manual operation. We evaluated the performance of an automated haematology analyser, the CELL-DYN Sapphire (CD Sapphire; Abbott Laboratories, USA) for T-lymphocyte subset analysis. METHODS: The precision and linearity obtained using the CD Sapphire was evaluated. T-lymphocyte subsets in blood samples from 120 patients were quantified using CD Sapphire and flow cytometry (Cytomics FC 500; Beckman-Coulter, France). The time required for complete T cell subset analysis using both methods was also evaluated. RESULTS: Results of CD Sapphire-based quantitation of CD3+, CD3+CD4+, and CD3+CD8+ cells showed intra-assay CV of less than 5% for precision and displayed linearity in the ranges of 84 to 5364, 41 to 2615, and 44 to 2800 cells/microL, respectively. There was good correlation among the CD3+, CD3+CD4+, and CD3+CD8+ cell counts as well as in the CD4/CD8 ratio (r=0.987, 0.982, 0.982, and 0.980, respectively) using CD Sapphire and flow cytometry. The mean turnaround time for the CD Sapphire (10.0+/-0.5 minutes) was significantly less than that for flow cytometry (111.8+/-8.4 minutes, P<0.001). CONCLUSIONS: T cell subset analysis using the CD Sapphire gives excellent performance and consistent results that correlate well with those obtained by flow cytometry. We conclude that this time-efficient method can replace conventional flow cytometric methods used for measuring T cell subsets.
