The Effects of Maturation Resistant Donor Dendritic Cells on Alloimmune Response in Mice.
- Author:
Jung Eun LEE
1
;
Hee Kyung KANG
;
Eun Young SEOUNG
;
Sung Hee YANG
;
Su Jin KIM
;
Sung Jun SHIN
;
Yon Su KIM
;
Jung Sang LEE
;
Suhnggwon KIM
Author Information
1. Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea. skimim@plaza.snu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Dendritic cells;
Transplantation tolerance;
Antigen presenting cells;
Clonal anergy
- MeSH:
Animals;
Antigen-Presenting Cells;
Cell Division;
Cell Proliferation;
Clonal Anergy;
Clone Cells;
Dendritic Cells*;
Graft Survival;
Humans;
Islets of Langerhans Transplantation;
Mice*;
Mice, Inbred C3H;
Phenotype;
Skin;
T-Lymphocytes;
Tissue Donors*;
Transplantation Tolerance;
Transplants
- From:Korean Journal of Nephrology
2005;24(4):514-525
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Although dendritic cells (DCs) are the most influential antigen presenting cells maturation of DC is the critical control point toward either activation or regulation of immunity. We hypothesized that pretreatment with donor DCs, if which were maturation-resistant in vivo, could enhance engraftment by inducing inactivated state for allo- reactive T cell clones. METHODS: Immature DCs were prepared by 6- day culture of BM cells and we used paraformaldehyde for locking the DCs as immature phenotypes. We did in vitro and in vivo MLR to evaluate the effect of maturation resistant DCs on alloreactive T cells and we confirmed the effect of DCs in MHC full mismatched skin and islet transplantation model. RESULTS: Fixed DCs in immature state were resistant to maturation stimuli and weak stimulator for allo-reactive T cells (CB6F1-->C3H). In contrast, fixed DCs in mature state stimulated allogeneic T cell proliferation effectively. Splenocytes isolated from mice 2 weeks after maturation resistant DC injection could not be reactivated and maintained naive phenotype when cocultured with allogeneic splenocytes (BALB/c-->C57BL6). Consistent with this finding maturation resistant DC treatment suppressed MLR-driven T cell division (CB6F1-->C3H) as assessed by CFSE analysis. But, CD25+ T cells depletion by treatment with anti-CD25 prior to DCs transfer attenuated this regulatory effect of DCs. In a MHC mismatched transplantation model (CB6F1-->C3H), treatment with maturation-resistant DCs 2 weeks before operation, markedly prolonged skin and islet graft survival. But C3H mice pretreated with CB6F1 DCs rejected DBA1 (H-2q) skin graft within 14 days. CONCLUSION: These findings suggest the maintenance of immaturity of DCs is a key factor in modulating alloimmune responses through dendritic cells.
