Biological Property of Recombinant Hemagglutinin-Neuraminidase Protein of Avian Paramyxovirus Type 6 Expressed by Recombinant Baculovirus.
10.4167/jbv.2015.45.4.319
- Author:
Ji Ye KIM
1
;
Hyun Jeong LEE
;
Soo Jeong KYE
;
Saeromi KIM
;
Hee Jung SEUL
;
Sang Eun KIM
;
Hee Soo LEE
;
Suk Chan JUNG
;
Kang Seuk CHOI
Author Information
1. Avian Diseases Division, Animal and Plant Quarantine Agency, Gyeonggi, Korea. kchoi0608@korea.kr
- Publication Type:Original Article
- Keywords:
Avian paramyxovirus-6;
Hemagglutinin-neuraminidase protein;
Baculovirus expression system;
Hemagglutination inhibition test
- MeSH:
Avulavirus*;
Baculoviridae*;
Blotting, Western;
Clone Cells;
Cross Reactions;
Diagnosis;
Ducks;
Glycosylation;
Hemagglutination;
HN Protein;
Immune Sera;
Insects;
N-Acetylneuraminic Acid;
Serologic Tests;
Serotyping;
Spodoptera
- From:Journal of Bacteriology and Virology
2015;45(4):319-327
- CountryRepublic of Korea
- Language:English
-
Abstract:
Hemagglutination inhibition (HI) test employing whole virus antigen is a prescribed serological test for serotyping, diagnosis and surveillance for avian paramyxoviruses (APMVs). For use as alternative to the virus antigen, hemagglutinin-neuraminidase (HN) protein gene of the wild duck isolate APMV-6/WB12-163FS of APMV serotype 6 (APMV-6) was amplified, cloned and expressed in Spodoptera frugiperda insect cells. The HN gene of 1,842 bps in length showed nucleotide and amino acid homology of 93.4% and 97.1%, respectively with that of APMV-6 prototype strain. Putative sialic acid binding motif and potential N-linked glycosylation sites were conserved. In Western blot analysis, the expressed protein had a molecular mass of 66 kDa and reacted specifically with antiserum to APMV-6. In addition, the recombinant HN protein showed biological properties such as hemagglutination (HA) and elution. The recombinant HN protein produced from infected cells showed high HA titers (approximately 2(13) HA unit/ml). The HA activity of the recombinant HN protein was inhibited by antisera to APMV-6. In cross HA inhibition test, the recombinant HN protein had the highest titers with antisera to homologous APMV serotype, although there was weak cross reaction with some of antisera to other APMV serotypes. Our results indicated that recombinant APMV-6 HN protein would have the potential as alternative to the APMV-6 antigen in HI assays.
