Preliminary Study of Tissue-Engineered Ileal Conduit Using Poly (epsilon-Caprolactone) (PCL) Nano-Sheet Seeded with Muscle-Derived Stem Cells.
10.4111/kju.2009.50.3.282
- Author:
Yong Seok LEE
1
;
Soo Yeon CHO
;
Hyun Woo KIM
;
Se Hee KANG
;
Hee Youn KIM
;
Ji Young LEE
;
Eun Bi KWON
;
Song Karp HONG
;
Ji Youl LEE
Author Information
1. Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea. uroljy@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Urinary diversion;
Tissue engineering;
Stem cells
- MeSH:
Animals;
Female;
Fluorescent Antibody Technique;
Humans;
Hypogonadism;
Indoles;
Mitochondrial Diseases;
Muscle, Skeletal;
Muscles;
Myogenin;
Myosin Heavy Chains;
Omentum;
Ophthalmoplegia;
Rats;
Rats, Sprague-Dawley;
Seeds;
Stem Cells;
Tissue Engineering;
Urinary Diversion
- From:Korean Journal of Urology
2009;50(3):282-287
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We studied the feasibility and results of a tissue-engineered ileal conduit using a poly (epsilon-caprolactone) (PCL) nano-sheet seeded with muscle-derived stem cells to replace a conventional ileal conduit in rats. MATERIALS AND METHODS: Muscle-derived stem cells were isolated from the gastrocnemius muscle of female Sprague-Dawley rats (200-250 g, n=6) by use of a preplate technique and were cultured on a PCL nano-sheet. The PCL nano-sheet was implanted into the omentum of rats and was then made into a conical shaped conduit. Rats were sacrificed 4 and 8 weeks after implantation, and morphologic changes were assessed by H&E and immunofluorescence staining, including DAPI staining and staining for myogenin and myosin heavy chain (MyHC). RESULTS: All rats survived until the end of the experiment. A minimal inflammatory reaction was observed around the PCL nano-sheet in the 4 week specimens but was found to be reduced in the 8 week specimen. Muscle bundles were identified at week 4 as well as week 8 after implantation on H&E staining. Around the PCL sheet, immunostaining for both myogenin and MyHC were positive, indicating skeletal muscle differentiation and ingrowth into the PCL sheet. CONCLUSIONS: A PCL nano-sheet seeded with muscle-derived stem cells showed successful skeletal muscle differentiation at 4 and 8 weeks after implantation. This preliminary result supports the feasibility of a tissue-engineered ileal conduit using a PCL nano-sheet (seeded with muscle-derived stem cells) in place of conventional ileal conduits.
