Effects of Injection Therapy using Muscle Derived Stem Cell/Chitosan/Hydroapatite Composite Gel in a Rat Model of Urinary Incontinence.
10.4111/kju.2007.48.6.627
- Author:
Gwoan Youb CHOO
1
;
Ji Youl LEE
;
Won Hee PARK
;
Yong Sik JUNG
Author Information
1. Department of Urology, Inha University College of Medicine, Incheon, Korea. drwonhee@inha.ac.kr
- Publication Type:Original Article
- Keywords:
Muscle;
Stem cells;
Urinary incontinence;
Rats
- MeSH:
Animals;
Denervation;
Female;
Humans;
Models, Animal*;
Muscle, Skeletal;
Pudendal Nerve;
Rats*;
Stem Cells;
Urethra;
Urinary Incontinence*
- From:Korean Journal of Urology
2007;48(6):627-632
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We investigated whether periurethral injections of muscle- derived stem cells (MDSC) and chitosan/hydroapatite after denervation of rat's pudendal nerve could increase the leak point pressure over a long time period in a rat model of urinary incontinence. MATERIALS AND METHODS: Muscle-derived stem cells isolated from the gastrocnemius muscle of normal female rats were purified to obtain a myogenic population by using the preplate technique. The N group was the normal female rats, the D Group was the pudendal nerve transected group and the M Group was the MDSC/chitosan/hydroapatite composite gel injected group after pudendal nerve transection. The MDSC/chitosan/hydroapatite composite gel was injected into the proximal periurethral area. At 2 and 4 weeks, visually identified leak point pressure measurement was done with using the vertical tilt/intravesical pressure clamp model of urinary incontinence. The rats were then sacrificed and the periurethral tissues harvested for histological examination. RESULTS: The leak point pressure was significantly lower in the D group at each time compared with the N group, and the leak point pressure in the N and M groups were significantly higher than those in the D group at both 2 and 4 weeks. The persistence of MDSC over the period of study was verified by histological examination. CONCLUSIONS: MDSC/chitosan/hydroapatite injection into the denervated external urethral sphincter in female rats increased the leak point pressure at 2 and 4 weeks. This MDSC/chitosan/hydroapatite composite gel can be an alternative injection method for treating urinary incontinence in the future.
