The Relationship between HIF-2α and VEGF with Radiographic Severity in the Primary Osteoarthritic Knee.
10.3349/ymj.2016.57.3.735
- Author:
Zhou JIAN-LIN
1
;
Fang HONG-SONG
;
Peng HAO
;
Deng SHUANG
;
Chen SHEN
;
Li JIAN-PING
;
Qiu BO
;
Weng JIN-QING
;
Liu FENG
Author Information
1. Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China. zhoujianlin2005@sina.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
HIF-2α;
VEGF;
cartilage degeneration;
KL grade
- MeSH:
Adult;
Aged;
Arthroplasty, Replacement, Knee;
Arthroscopy;
Basic Helix-Loop-Helix Transcription Factors/*metabolism;
Biomarkers/*blood;
Cartilage/*metabolism;
Female;
Humans;
Knee Joint/*diagnostic imaging;
Male;
Middle Aged;
Osteoarthritis, Knee/*blood/diagnostic imaging/physiopathology;
RNA, Messenger;
Radiography;
Real-Time Polymerase Chain Reaction;
Severity of Illness Index;
Vascular Endothelial Growth Factor A/*metabolism
- From:Yonsei Medical Journal
2016;57(3):735-740
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The aim of this study was to determine the relationship of hypoxia-inducible factor-2 (HIF-2α) and vascular endothelial growth factor (VEGF) with radiographic severity in primary osteoarthritis (OA) of the knee. Expression of these two factors in cartilage samples from OA knee joints was examined at mRNA and protein levels. MATERIALS AND METHODS: Knee joints were examined using plain radiographs, and OA severity was assessed using the Kellgren and Lawrence (KL) grading system. Specimens were collected from 29 patients (31 knees) who underwent total knee replacement because of severe medial OA of the knee (KL grades 3 and 4), 16 patients who underwent knee arthroscopy (KL grade 2), and 5 patients with traumatic knees (KL grade 0). HIF-2α and VEGF expression was quantified by real-time polymerase chain reaction and western blotting. RESULTS: Cartilage degeneration correlated with the radiographic severity grade. OA severity, determined using the Mankin scale, correlated positively with the KL grade (r=0.8790, p<0.01), and HIF-2α and VEGF levels with the radiographic severity of knee OA (r=0.7001, p<0.05; r=0.6647, p<0.05). CONCLUSION: In OA cartilage, HIF-2α and VEGF mRNA and protein levels were significantly and positively correlated. The expression of both factors correlated positively with the KL grade. HIF-2α and VEGF, therefore, may serve as biochemical markers as well as potential therapeutic targets in knee OA.
