Oxidative damage biomarker levels according to Mn-SOD and GST gene polymorphisms in preschool children.
10.4163/jnh.2015.48.6.468
- Author:
You Kyung SHIN
1
;
Ji Won CHOI
;
Se Young OH
;
Jayong CHUNG
Author Information
1. Department of Food and Nutrition, Kyung Hee University, Seoul 02447, Korea. jchung@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Genetic polymorphism;
Mn-superoxide dismutase;
glutathione S-transferase;
8-hydroyxyl-2-deoxyguanosine
- MeSH:
Biomarkers;
Child;
Child, Preschool*;
DNA;
Enzyme-Linked Immunosorbent Assay;
Genotype;
Glutathione Transferase;
Gyeonggi-do;
Hand;
Humans;
Malondialdehyde;
Multiplex Polymerase Chain Reaction;
Oxidative Stress;
Plasma;
Polymorphism, Genetic;
Seoul;
Smoking;
Superoxide Dismutase*
- From:Journal of Nutrition and Health
2015;48(6):468-475
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Genetic polymorphisms in antioxidant defense and detoxification genes may modulate the levels of oxidative stress biomarkers. METHODS: A total of 301 healthy preschool-aged children in the Seoul and Kyung-gi areas were recruited. DNA was extracted from blood for genotyping of manganese superoxide dismutase (Mn-SOD) Val16Ala, glutathione S-transferase (GST) P1 Ile105Val, GSTT1 present/null, and GSTM1 present/null polymorphisms by PCR-restriction fragment length polymorphism or multiplex PCR analyses. In addition to a questionnaire survey, the levels of urinary 8- hydroxyl-2-deoxiguanosine (8-OHdG) and plasma malondialdehyde (MDA) were measured by ELISA. RESULTS: Significantly higher urinary 8-OHdG concentrations were observed in GSTP1 Ile/Val + Val/Val genotype (p = 0.030), and tended to be higher in Mn-SOD Val/Val genotype (p = 0.065). On the other hand, exposure to environmental tobacco smoking (ETS) and interaction between ETS and gene polymorphisms did not significantly influence either urinary 8-OHdG concentrations or serum MDA. CONCLUSION: Based on our findings, GSTP1 Ile/Val gene polymorphisms might modulate the levels of oxidative stress biomarkers in healthy preschool children.
