Alterations in NK Cell Cytotoxicity Induced by Peripheral Nerve Injury in Mice.
10.4097/kjae.2007.52.2.219
- Author:
You Jin KANG
1
;
Ho Kyung SONG
;
Jin Young CHON
;
Joo Hyun YOU
Author Information
1. Department of Anesthesiology and Pain Medicine, St. Vincent's Hospital, Suwon, Korea.
- Publication Type:Original Article
- Keywords:
immune system;
immunologic cytotoxicity test;
neuropathy;
NK cell;
pain
- MeSH:
Animals;
Cell Line;
Chronic Pain;
Cytotoxicity Tests, Immunologic;
Hyperalgesia;
Immune System;
Killer Cells, Natural*;
L-Lactate Dehydrogenase;
Mice*;
Neuralgia;
Peripheral Nerve Injuries*;
Peripheral Nerves*;
Prognosis;
Sciatic Nerve
- From:Korean Journal of Anesthesiology
2007;52(2):219-223
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Chronic pain is often associated with changes in the immune responses, which highlights the need for the aggressive pain control to obtain a better prognosis. This study examined splenic NK cell cytotoxicity in an attempt to assess the possible changes in the immune function under chronic neuropathic pain after a partial transsection of the sciatic nerve. METHODS: After confirming tactile allodynia in response to the von Frey filament, a modified lactate dehydrogenase (LDH) release assay was used to determine the cytotoxic activity of splenic NK cells on the YAC-1 cell line in C3H/HeN (H-2k) mice (n = 6). NK cells as effector cells were mixed with YAC-1 cells as target cells (1 x 10(4)/100microliter), resulting in an effector-target ratio of 1 : 25, 1 : 50, 1 : 100 in the culture medium. RESULTS: At 1 and 2 weeks after the nerve injury, all the subjects showed significant mechanical sensitivity compared with those observed before surgery. The percentage of NK cell cytotoxicity of the neuropathic mice increased significantly 1 week after the nerve injury but decreased within 2 weeks compared with the normal mice. CONCLUSIONS: In terms of the altered NK cell cytotoxicity, neuropathic pain can cause changes in the normal performance of the immune function.
