Inhibitory Effect of Rubus Coreanus on Compound 48/80- or Anti-DNP IgE-Induced Mast Cell Activation.
- Author:
Guang Zhao LI
1
;
Ok Hee CHAI
;
Chang Ho SONG
Author Information
- Publication Type:Original Article
- Keywords: Rubus coreanus; mast cell; histamine; calcium; cAMP
- MeSH: Animals; Calcium; Fruit; Histamine; Histamine Release; Mast Cells*; Rats; Vegetables
- From:Immune Network 2004;4(2):100-107
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: The fruit of Rubus coreanus (RC), a perennial herb, has been cultivated for a long time as a popular vegetable. The anti-allergy mechanism of RC is unknown. The purpose of this study is to investigate the inhibitory effect of RC on compound 48/80- or anti-DNP IgE-induced mast cell activation. METHODS: For this, influences of RC on the compound 48/80-induced degranulation, histamine release, calcium influx and the change of the intracellular cAMP (cyclic adenosine-3',5' monophosphate) levels of rat peritoneal mast cells (RPMC) and on the anti-DNP IgE-induced histamine release of RPMC were observed. RESULTS: The pretreatment of RC inhibited compound 48/ 80-induced degranulation, histamine release and intracelluar calcium uptake of RPMC. The anti-DNP IgE-induced histamine release of RPMC was significantly inhibited by pretreatment of RC. The RC increased the level of intracellular cAMP of RPMC, and the pretreatment of RC inhibited compound 48/80-induced decrement of intracellular cAMP of RPMC. CONCLUSION: These results suggest that RC contains some substances with an activity to inhibit the compound 48/80- or anti-DNP IgE-induced mast cell activitation. The inhibitory effects of RC are likely due to the stabilization of mast cells by blocking the calcium uptake and enhancing the level of intracellular cAMP.
