Beta ig-h3 promotes renal proximal tubular epithelial cell adhesion, migration and proliferation through the interaction with alpha3beta1 integrin.
- Author:
Sun Woo PARK
1
;
Jong Sup BAE
;
Ki San KIM
;
Sun Hee PARK
;
Byung Heon LEE
;
Je Yong CHOI
;
Jae Yong PARK
;
Sung Woo HA
;
Yong Lim KIM
;
Tae Hwan KWON
;
In San KIM
;
Rang Woon PARK
Author Information
1. Cell and Matrix Biology National Research Laboratory, Department of Biochemistry.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
adhesion;
alpha3beta1 integrin;
betaig-h3;
migration;
proliferation;
renal proximal tubular epithelial cell
- MeSH:
Amino Acid Motifs;
Antibodies, Blocking/immunology;
Cell Adhesion/physiology;
Cell Movement/physiology;
Cell Proliferation;
Cells, Cultured;
Epithelial Cells/drug effects;
Extracellular Matrix Proteins/chemistry/immunology/*metabolism;
Humans;
Integrin alpha3beta1/chemistry/immunology/*metabolism;
Kidney Tubules, Proximal/cytology/metabolism/*physiology;
Peptides/chemistry/metabolism;
Protein Interaction Mapping;
Research Support, Non-U.S. Gov't;
Transforming Growth Factor beta/chemistry/immunology/*metabolism/pharmacology
- From:Experimental & Molecular Medicine
2004;36(3):211-219
- CountryRepublic of Korea
- Language:English
-
Abstract:
Betaig-h3 (betaig-h3) is a secretory protein composed of fasciclin I-like repeats containing sequences that allows binding of integrins and glycosaminoglycans in vivo. Expression of betaig-h3 is responsive to TGF-beta and the protein is found to be associated with extracellular matrix (ECM) molecules, implicating betaig-h3 as an ECM adhesive protein of developmental processes. We previously observed predominant expression of betaig-h3 expression in the basement membrane of proximal tubules of kidney. In this study, the physiological relevance of such localized expression of betaig-h3 was examined in the renal proximal tubular epithelial cells (RPTEC). RPTEC constitutively expressed betaig-h3 and the expression was dramatically induced by exogenous TGF-beta1 treatment. betaig-h3 and its second and fourth FAS1 domain were able to mediate RPTEC adhesion, spreading and migration. Two known alpha3beta1 integrin-interaction motifs including aspartatic acid and isoleucine residues, NKDIL and EPDIM in betaig-h3 were responsible to mediate RPTEC adhesion, spreading, and migration. By using specific antibodies against integrins, we confirmed that alpha3beta1 integrin mediates the adhesion and migration of RPTECs on betaig-h3. In addition, it also enhanced proliferation of RPTECs through NKDIL and EPDIM. These results indicate that betaig-h3 mediates adhesion, spreading, migration and proliferation of RPTECs through the interaction with alpha3beta1 integrin and is intimately involved in the maintenance and the regeneration of renal proximal tubular epithelium.
