Standardization of Isolation Procedure and Analysis of Variables on Successful Isolation of Islet from the Human Pancreas.
10.3803/jkes.2006.21.1.22
- Author:
Song Cheol KIM
1
;
Duck Jong HAN
;
Ik Hee KIM
;
Yoo Me WE
;
Yang Hee KIM
;
Jin Hee KIM
;
Ji He BACK
;
Dong Gyun LIM
Author Information
1. Department of Surgery, Ulsan University College of Medicine and Asan Medical Center, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Islet transplantation;
Islet isolation;
Human pancreas
- MeSH:
Cadaver;
Cold Ischemia;
Collagenases;
Digestion;
Humans*;
Islets of Langerhans Transplantation;
Logistic Models;
Pancreas*;
Tissue Donors
- From:Journal of Korean Society of Endocrinology
2006;21(1):22-31
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Identifying the donor and isolation-related factors during the islet isolation would be greatly helpful to improve the result of human islet isolation for successful clinical islet transplantation. METHODS: Sixty-nine pancreata from cadaveric donors were isolated with standard protocol and analyzed to identify the donor factors and isolation variables for successful isolation. Islet isolations recovered > or = 100,000 Islet Equivalent (IEQ, n=53) were compared to islet mass less than 100,000 IEQ (n=16). RESULTS: The mean islet recovery was 216.0 x 10(3) +/- 173.7 x 10(3) (IEQ) before purification and 130.6 x 10(3) +/- 140.2 x 10(3) (IEQ) after purification. Mean purity was 54 +/- 31%. Mean age of donor was 31.2 +/- 13.2 year and mean cold ischemic time was 6.9 +/- 6.2 hour. Quality of isolated islets was acceptable in terms of bacterial culture, viability and secretory function in vitro and in vivo. In univariate analysis on successful isolation, status of pancreas was the only significant factor and sex, duration of collagenase expansion and digestion time were marginal factors. Stepwise multivariate logistic regression analysis showed donor sex, status of pancreas and digestion time were significant factors for the successful islet isolation. CONCLUSION: This study confirms some donor factors and variables in isolation process can influence the ability to obtain the successful isolation of human islet. Enough experiences and pertinent review of donor and isolation factors can make islet isolation successful, supporting the clinical islet transplantation without spending of cost.
