The Effect of TGF-beta1 on Cellular Activity of Periodontal Ligament Cells activated by PDGF-BB.
10.5051/jkape.2002.32.3.457
- Author:
Sang Churl BAEK
1
;
Jin Woo PARK
;
Jo Young SUH
Author Information
1. Department of Periodontology, College of Dentistry Kyungpook National University, Korea.
- Publication Type:In Vitro ; Original Article
- MeSH:
Bicuspid;
Collagen;
DNA;
Humans;
Incubators;
Mitogens;
Periodontal Ligament*;
Regeneration;
Tooth;
Transforming Growth Factor beta1*
- From:The Journal of the Korean Academy of Periodontology
2002;32(3):457-473
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The purposes of this study is to evaluate the combination effects of TGF-beta1 and PDGF-BB on the periodontal ligament cells to use as a regeneration promoting agent of periodontal tissue. Human periodontal ligament cells were prepared from the first premolar tooth extracted for the orthodontic treatment and were cultured in DMEM/10% FBS at the 37degrees C, 5% CO2 incubator. Authors measured the DNA synthesis, total protein, collagen and noncollagenous protein synthesis according to the concentration of TGF-beta1, (1, 5ng/ml) and PDGF-BB (1, 10 ng/ml) in combination. To explore further this delayed effect of TGF-beta1, we preincubated human periodontal ligament cells with TGF-beta1 for 4 or 24 hours before PDGF-BB stimulation. The results were as follows: The DNA synthetic activity was increased dose dependently by TGF-beta1, PDGF-BB. The combination of TGF-beta1 and PDGF-BB consistently enhanced the DNA synthetic activity to PDGF-BB alone. The ability of TGF-beta1 to enhance DNA synthetic activity in PDGF-BB treated periodontal ligament cells was dose dependent. The maximum mitogenic effect was at the 5ng/ml of TGF-beta1 and 10ng/ml of PDGF-BB. Preincubation of cells with TGF-beta1 resulted in significantly greater response to PDGF-BB at all TGF-beta1 concentration studied, and may be useful for clinical application in periodontal regenerative procedures. The total protein, collagen and noncollagen synthesis was increased dose pendently by TGF-beta1, PDGF-BB. The % of collagen was slightly decresed according to the concentration of TGF-beta1, PDGF-BB. The effect of TGF-beta1, PDGF-BB were not specific for collagen synthesis since it also increased noncollagenous protein synthesis. This study demonstrates that PDGF-BB is major mitogens for human periodontal ligament cells in vitro, and supports a role for TGF-beta1 as a regulation of the mitogenic and total protein formation to PDGF-BB in these cells.
