Effects of Topically Applied Autologous Serum on Aqueous Biochemistry and Endothelial Healing Following Experimental Corneal Alkali Wounds.
- Author:
Jung Won YOO
1
;
Jnag Hyun CHUNG
Author Information
1. Deapartment of Ophthalmology, Ewha Medical Research Center, Ewha University, Seoul, Korea.
- Publication Type:Original Article
- MeSH:
Alkalies*;
Aqueous Humor;
Ascorbic Acid;
Biochemistry*;
Cornea;
Endothelium;
Glucose;
Minerals;
Rabbits;
Wounds and Injuries*
- From:Journal of the Korean Ophthalmological Society
1998;39(9):1996-2002
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The effect of topically instilled autolohous serum on the healing of endothelium and change of aqueous chemical properties following alkali wounds were evaluated. New Zealand white rabbits, weighing 2.5KG, total 64(64 eyes), were used in this experiment. Standardized corneal alkali wounds were produced by applying a round filter paper of 5.5mm if diameter, soaked in 1N NaOH, to the central cornea for 60 seconds. Autologous serum in the treated group and balanced salt solution (BSS) in the control group were given 4 times per dat for 3 weeks. Endothelial defect areas and the concentrations of ascorbic acid, glucose and minerals in the aqueous humor were measured. Endothelial defect area was not significantly different between autologous serum-treated and control groups. The concentrations of ascorbic acid in the aqueous humor were aignificantly increased in the autologous serum-treated groups compared to the control groups. However, the concentrations of glucose, Na+, K+, Ca++ and Mg++ in the aqueous humor were not statistically different between two groups.
