Sensitivity and Specificity of Vi-Indirect Fluore scent Antibody Test in Diagnosis of Typhoid Fever.
- Author:
Kyung Hee CHANG
1
;
Dong Yeul RHYOO
;
Sung Ha PARK
;
Byung Kyu PARK
;
Joon Ku LEE
;
Jun Sup YEOM
;
Young Hwa CHOI
;
Young Gu SONG
;
Hyun Sook KIM
;
Yun Sop CHONG
;
June Myung KIM
Author Information
1. Department of Internal Medicine, College of Medicine, Yonsei University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Vi-antigen;
Typhoid fever;
Immunofluorescent antibody test
- MeSH:
Body Fluids;
Diagnosis*;
Early Diagnosis;
Fever;
Retrospective Studies;
Sensitivity and Specificity*;
Serologic Tests;
Typhoid Fever*
- From:Korean Journal of Infectious Diseases
1998;30(4):379-384
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Typhoid fever is diagnosed by culture or serological study. The confirmative diagnosis of typhoid fever is made by culture of the causative orga-nism usually from body fluids. Serological test is a supportive diagnostic tool, which is useful for early dia-gnosis. In Severance Hospital, Vi-indirect fluorescent antibody test(Vi-IFAT) using the Vi-antigen of Salmo-nella typhi has been used in the diagnosis of typhoid fever since 1989. We investigated the test results from the past 7 years, in order to clarify the sensitivity and specificity of Vi-IFAT. METHODS: A retrospective study was done on pa-tients whose chief complaint was fever and who were tested using Vi-IFAT in the Severance Hospital from 1989 to 1996. The positive value for Vi-IFAT was de- fined as 1:64 or higher. RESULTS: The sensitivity and specificity of Vi-IFAT for typhoid fever was 94.4% and 95.1%, respectively. The positive and negative predictive values were 85.7% and 98.2% respectively. Positive rates of Vi-IFAT after fever onset increased with time and 68% were positive before the first week. From the first to the second week, 89.5% were positive and after the second week, 100% were positive. CONCLUSION: Vi-IFAT is not only a valuable sero-logic test for the diagnosis of typhoid fever, but also useful in the early diagnosis of the disease.
