Immunoreactivity of CD99 in Stomach Cancer.
10.3346/jkms.2002.17.4.483
- Author:
Kyeong Cheon JUNG
1
;
Weon Seo PARK
;
Young Mee BAE
;
Jang Hee HAHN
;
Kyuhyoung HAHN
;
Hansoo LEE
;
Hae Wan LEE
;
Hyung Jin KOO
;
Hai Jeong SHIN
;
Hyung Sik SHIN
;
Young Euy PARK
;
Seong Hoe PARK
Author Information
1. Department of Pathology, College of Medicine, Hallym University, Chunchon, Korea. jungkc@hallym.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
CD99;
Stomach Neoplasms;
Adenocarcinoma;
Matrix Metalloproteinases
- MeSH:
Adenocarcinoma/*immunology/pathology;
Adult;
Aged;
Antigens, CD/*analysis/genetics;
Cell Adhesion Molecules/*analysis/genetics;
Female;
Gastric Mucosa/cytology/immunology;
Humans;
Male;
Matrix Metalloproteinases/metabolism;
Middle Aged;
Protein Isoforms/analysis/genetics;
RNA, Messenger/genetics/metabolism;
Stomach Neoplasms/*immunology/pathology
- From:Journal of Korean Medical Science
2002;17(4):483-489
- CountryRepublic of Korea
- Language:English
-
Abstract:
CD99 is characteristically expressed in Ewing's sarcoma/primitive neuroectodermal tumor. Recently its immunoreactivity has also been reported in other tumors. However, the significance of CD99 isoforms expressed in these tumors has not been elucidated. In this study, we evaluated the expression of CD99 isoforms and its relationship with histopathologic parameters in gastric adenocarcinomas. Paraffin sections of 46 gastric adenocarcinomas were stained with an anti-CD99 monoclonal antibody, YG32. Twelve (26.1%) cases of 46 gastric adenocarcinomas showed immunoreactivity to YG32. The CD99 expression was also seen both in non-neoplastic foveolar epithelial cells and infiltrating lymphocytes. In addition, Western blot and RT-PCR analyses revealed that the type I is the predominant isoform of CD99 in non-neoplastic and neoplastic gastric tissues. The CD99 expression was usually seen in the intestinal type adenocarcinoma, while rarely in the diffuse type. The CD99 immunoreactivity decreased in MMP-2-overexpressing adenocarcinomas (p=0.028). Our results suggest that the type I is the major isoform of CD99 expressed in non-neoplastic gastric mucosa and gastric adenocarcinomas and its downregulation in gastric adenocarcinoma may be associated with cellular dedifferentiation and/or MMP-2 overexpression.
