Prednisolone and Glucose-6-phosphatase Activity in Liver Cells.
- Author:
Tai Sun SHIN
1
;
In Hyuk CHUNG
;
Soo Sung KIM
Author Information
- Publication Type:Original Article
- MeSH: Animal; Glucose-6-Phosphatase/analysis*; Liver/drug effects; Liver/enzymology*; Male; Prednisolone/pharmacology*; Rats
- From:Yonsei Medical Journal 1977;18(1):9-18
- CountryRepublic of Korea
- Language:English
- Abstract: Prednisolone, a cortisol analogue, was given intraperitoneally to rats with 5, 10 or 15 doses of 5 mg. per kg. of body weight per day. Sacrificing the animals 24 hours after the last injection, unfixed frozen sections from small pieces of liver tissue were incubated and stained by a modification from the method of Wachstein and Meisel(1965) for glucose-6-phosphatase (G-6-Pase) activity. Some of the tissue blocks were processed for staining with hematoxylin and eosin for histopathological observation. Glucose-6-phosphatase activity, being represented histochemically by brownish-black deposits, was progressively increased after administrations 5 or 10 times. With 15 doses of prednisolone the general histochemical picture of G-6-Pase activity appeared to be similar to that of the control group, except for a different distribution of hepatocytes possessing strong activity. In prednisolone treated rats, the swollen hepatocytes showed a marked, cytoplasmic vacuolization and nuclear pyknosis, particularly in the periportal and midzonal areas of hepatic lobules. Some discussion of the G-6-Pase corticosteroids are presented in terms of their metabolic effects.
