Increased Cytokine and Nitric Oxide Levels in Serum of Dogs Experimentally Infected with Rangelia vitalii.
10.3347/kjp.2013.51.1.133
- Author:
Francine C PAIM
1
;
Aleksandro S DA SILVA
;
Carlos Breno V PAIM
;
Raqueli T FRANCA
;
Marcio M COSTA
;
Marta M M F DUARTE
;
Manuela B SANGOI
;
Rafael N MORESCO
;
Silvia G MONTEIRO
;
Sonia Terezinha A LOPES
Author Information
1. Laboratory of Veterinary Clinical Analysis-LACVet, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. franchimelo@gmail.com
- Publication Type:Brief Communication
- Keywords:
Rangelia vitalii;
IFN-gamma;
TNF-alpha;
IL-1;
IL-6;
nitric oxide;
immunity;
rangeliosis
- MeSH:
Animals;
Chemistry Techniques, Analytical;
Cytokines/*blood;
Disease Models, Animal;
Dogs;
Enzyme-Linked Immunosorbent Assay;
Female;
Nitric Oxide/*blood;
Piroplasmida/*immunology;
Protozoan Infections/*immunology/parasitology/pathology;
Serum/chemistry
- From:The Korean Journal of Parasitology
2013;51(1):133-137
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study aimed to measure the levels of interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), interleukin 1 (IL-1), interleukin 6 (IL-6), and nitrite/nitrate (NOx) in serum of dogs experimentally infected with Rangelia vitalii. Twelve female mongrel dogs were divided into 2 groups; group A (uninfected controls) composed by healthy dogs (n=5) and group B consisting of dogs inoculated with R. vitalii (n=7). Animals were monitored by blood smear examinations, which showed intraerythrocytic forms of the parasite on day 5 post-infection (PI). Blood samples were collected through the jugular vein on days 0, 10, and 20 PI to determine the serum levels of IFN-gamma, TNF-alpha, IL-1, IL-6, and NOx. Cytokines were assessed by ELISA quantitative sandwich technique, and NOx was measured by the modified Griess method. Cytokine levels (IFN-gamma, TNF-alpha, IL-1, and IL-6) were increased (P<0.01) in serum of infected animals. Serum levels of NOx were also increased on days 10 PI (P<0.01) and 20 PI (P<0.05) in infected animals. Therefore, the infection with R. vitalii causes an increase in proinflammatory cytokines and nitric oxide content. These alterations may be associated with host immune protection against the parasite.
