Quantitative Analysis of Eleven Bioactive Constituents of a Traditional Herbal Medicine, Yeonggyechulgam-tang using, Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry.
10.20307/nps.2017.23.2.84
- Author:
Chang Seob SEO
1
;
Hyeun Kyoo SHIN
Author Information
1. K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Korea. hkshin@kiom.re.kr
- Publication Type:Original Article
- Keywords:
Quantitative analysis;
Yeonggyechulgam-tang;
LC-MS/MS
- MeSH:
Calibration;
Glycyrrhizic Acid;
Herbal Medicine*;
Limit of Detection;
Methods;
Tandem Mass Spectrometry*;
Water
- From:Natural Product Sciences
2017;23(2):84-91
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study proposes a sensitive and selective liquid chromatography-electrospray ionization tandem mass spectrometry method of efficiently assessing the quality of a traditional herbal medicine called Yeonggyechulgam-tang (YGCGT). The following compounds 1 – 11, namely, liquiritin apioside (1), liquiritin (2), liquiritigene (3), coumarin (4), cinnamic acid (5), cinnamaldehyde (6), glycyrrhizin (7), atractylenolide III (8), atractylenolide II (9), atractylenolide I (10), and pachymic acid (11) were separated on a UPLC BEH C₁₈ column (2.1 × 100 mm, 1.7 µm) at a column temperature of 45℃ eluted with a gradient condition of 0.1% (v/v) formic acid in distilled water and acetonitrile. The correlation coefficient of the calibration curve of the eleven constituents was ≥ 0.9936. The limits of detection and quantification of the compounds 1 – 11 were 0.06 – 4.73 ng/mL and 0.17–14.20 ng/mL, respectively. Using this analytical method, the compound 11 in lyophilized YGCGT decoction extract was not detected, while the compounds 1 – 10 were detected 0.13–166.43 mg/g.
