Inhibitory Effect of a Sesquiterpene from Artemisia iwayomogi on Expression of Inducible Nitric Oxide Synthase by Suppression of I-κBα Degradation in LPS-stimulated RAW 264.7 Cells.
10.20307/nps.2017.23.2.92
- Author:
Na Yeon KIM
1
;
Hye Jin KOH
;
Hua LI
;
Hwa Jin LEE
;
Jae Ha RYU
Author Information
1. College of Pharmacy, Sookmyung Women's University, Seoul 04310, Republic of Korea. ryuha@sookmyung.ac.kr
- Publication Type:Original Article
- Keywords:
Artemisia iwayomogi;
Armefolin;
Sesquiterpene;
Nitric oxide;
Inducible nitric oxide synthase
- MeSH:
Artemisia*;
Cytokines;
Interleukin-6;
Macrophages;
Methanol;
Nitric Oxide;
Nitric Oxide Synthase Type II*;
Plants, Medicinal;
RAW 264.7 Cells*;
RNA, Messenger
- From:Natural Product Sciences
2017;23(2):92-96
- CountryRepublic of Korea
- Language:English
-
Abstract:
A sesquiterpene was purified from Artemisia iwayomogi methanolic extract during the course of searching anti-inflammatory principle from medicinal plants. A sesquiterpene identified as armefolin inhibited the production of nitric oxide (NO) and attenuated inducible nitric oxide synthase (iNOS) protein level in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Armefolin also down-regulated mRNA expressions of iNOS and pro-inflammatory cytokines, interleukin-1β and interleukin-6 in LPS-activated macrophages. Moreover, armefolin suppressed the degradation of inhibitory-κBα (I-κBα) in LPS-activated macrophages. These data suggest that armefolin from A. iwayomogi can suppress the LPS-induced production of NO and the expression of iNOS gene through inhibiting the degradation of I-κBα. Taken together, armefolin from A. iwayomogi might be a candidate as promising anti-inflammatory agent.
