Effects of Transglutaminase 2 Inhibition on Ventilator-Induced Lung Injury.
10.3346/jkms.2014.29.4.556
- Author:
In Bum SUH
1
;
Dae Wui YOON
;
Won Oak OH
;
Eun Joo LEE
;
Kyung Hoon MIN
;
Gyu Young HUR
;
Seung Heon LEE
;
Sung Yong LEE
;
Sang Yeub LEE
;
Chol SHIN
;
Jae Jeong SHIM
;
Kwang Ho IN
;
Kyung Ho KANG
;
Je Hyeong KIM
Author Information
1. Department of Laboratory Medicine, College of Medicine, Kangwon National University, Chuncheon, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Acute Lung Injury;
Respiratory Distress Syndrome, Adult;
Respiration, Artificial;
Ventilator-Induced Lung Injury;
Inflammation;
Transglutaminase 2
- MeSH:
Acute Lung Injury/pathology;
Animals;
Cystamine/therapeutic use;
Cytokines/analysis;
Enzyme Inhibitors/therapeutic use;
Enzyme-Linked Immunosorbent Assay;
GTP-Binding Proteins/*antagonists & inhibitors/genetics/metabolism;
Gene Expression;
Lipopolysaccharides/toxicity;
Male;
Mice;
Mice, Inbred C57BL;
NF-kappa B/metabolism;
Respiration, Artificial;
Transglutaminases/*antagonists & inhibitors/genetics/metabolism;
Ventilator-Induced Lung Injury/*enzymology/pathology/prevention & control
- From:Journal of Korean Medical Science
2014;29(4):556-563
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was performed to examine the role of transglutaminase 2 (TG2) in ventilator-induced lung injury (VILI). C57BL/6 mice were divided into six experimental groups: 1) control group; 2) lipopolysaccharide (LPS) group; 3) lung protective ventilation (LPV) group; 4) VILI group; 5) VILI with cystamine, a TG2 inhibitor, pretreatment (Cyst+VILI) group; and 6) LPV with cystamine pretreatment (Cyst+LPV) group. Acute lung injury (ALI) score, TG2 activity and gene expression, inflammatory cytokines, and nuclear factor-kappaB (NF-kappaB) activity were measured. TG2 activity and gene expression were significantly increased in the VILI group (P < 0.05). Cystamine pretreatment significantly decreased TG2 activity and gene expression in the Cyst+VILI group (P < 0.05). Inflammatory cytokines were higher in the VILI group than in the LPS and LPV groups (P < 0.05), and significantly lower in the Cyst+VILI group than the VILI group (P < 0.05). NF-kappaB activity was increased in the VILI group compared with the LPS and LPV groups (P < 0.05), and significantly decreased in the Cyst+VILI group compared to the VILI group (P = 0.029). The ALI score of the Cyst+VILI group was lower than the VILI group, but the difference was not statistically significant (P = 0.105). These results suggest potential roles of TG2 in the pathogenesis of VILI.
