Proteomic Profiling of Serum from Patients with Tuberculosis.
10.3343/alm.2014.34.5.345
- Author:
Sang Hoon SONG
1
;
Minje HAN
;
Yang Seon CHOI
;
Ki Soon DAN
;
Man Gil YANG
;
Junghan SONG
;
Sung Sup PARK
;
Jae Ho LEE
Author Information
1. Clinical Proteomics Laboratory, Seoul National University Hospital, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Tuberculosis;
Proteomics;
Alpha-1-antitrypsin
- MeSH:
Adult;
Aged;
Antithrombin III/analysis;
Biological Markers/blood;
Chromatography, High Pressure Liquid;
Discriminant Analysis;
Female;
Humans;
Male;
Middle Aged;
Multivariate Analysis;
Proteome/*analysis;
*Proteomics;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;
Tuberculosis/*blood/genetics/metabolism;
alpha 1-Antitrypsin/analysis
- From:Annals of Laboratory Medicine
2014;34(5):345-353
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Effective treatment and monitoring of tuberculosis (TB) requires biomarkers that can be easily evaluated in blood samples. The aim of this study was to analyze the serum proteome of patients with TB and to identify protein biomarkers for TB. METHODS: Serum samples from 26 TB patients and 31 controls were analyzed by using nano-flow ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry in data-independent mode, and protein and peptide amounts were calculated by using a label-free quantitative approach. The generated data were analyzed by using principal component analysis and partial least squares discriminant analysis, a multivariate statistical method. RESULTS: Of more than 500 proteins identified, alpha-1-antitrypsin was the most discriminative, which was 4.4 times higher in TB patients than in controls. Peptides from alpha-1-antitrypsin and antithrombin III increased in TB patients and showed a high variable importance in the projection scores and coefficient in partial least square discriminant analysis. CONCLUSIONS: Sera from patients with TB had higher alpha-1-antitrypsin levels than sera from control participants. Alpha-1-antitrypsin levels may aid in the diagnosis of TB.
