Requirement of CD4 Help for Induction of CD8 T Cell Response Specific for Virally Derived H60.
- Author:
Su Jeong RYU
1
;
Bora KANG
;
Seok Ho KIM
;
Tae Woo KIM
;
Jun CHANG
;
Eun Young CHOI
Author Information
- Publication Type:Brief Communication
- Keywords: Recombinant vaccinia virus; Viral epitope; CD4 help; CD8 T cell response
- MeSH: Animals; Immunization; Mice; T-Lymphocytes; Vaccinia virus
- From:Immune Network 2012;12(3):118-125
- CountryRepublic of Korea
- Language:English
- Abstract: CD40-CD40L-mediated help from CD4 T cells is essential to induce primary CD8 T cell responses specific to the non-inflammatory cell-based antigen H60. In this study, using H60 as a model antigen, we generated recombinant vaccinia viruses (rVVs) expressing the H60 CD8 epitope and investigated whether CD4 help was required to activate the CD8 T cell response specific to the virally expressed H60. The immune response after infection with rVVs expressing H60 was similar to that after immunization with H60 congenic splenocytes, with a peak frequency of H60-specific CD8 T cells detected in the blood on day 10 post-infection. A CD8 T cell response specific for virally derived H60 was not induced in CD4-depleted mice, but was in CD40-deficient mice. These results provide insights into the characterization of the CD8 T cell response specifically for antigens originating from cellular sources compared to viral sources.
