Gene Frequencies of Human Neutrophil Antigens 4a and 5a in the Korean Population.
10.3343/kjlm.2006.26.2.114
- Author:
Tae Hee HAN
1
;
Kyou Sup HAN
Author Information
1. Department of Laboratory Medicine, Inje University Sanggyep Paik Hospital1, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
HNA-4a antigen;
HNA-5a antigen;
Gene frequency
- MeSH:
Gene Frequency*;
Granulocytes;
Homozygote;
Humans*;
Neutropenia;
Neutrophils*;
Polymerase Chain Reaction;
Restriction Mapping;
Tissue Donors
- From:The Korean Journal of Laboratory Medicine
2006;26(2):114-118
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: In Korean population, antigen frequencies of HNA-1a, HNA-1b, and HNA-2a are determined using a serological and genotyping method. However, no study has been done to assess the gene frequencies of HNA-4a and HNA-5a. It has been reported that the antibody against HNA-4a is associated with alloimmune neutropenia and autoimune neutropenia; however, there is no confirmed clinical report on anti-HNA-5a-related disorders. The aim of this study was to determine HNA-4a and HNA-5a gene frequencies among the Korean population. METHODS: Genotyping of HNA-4a and HNA-5a genes of 110 healthy and unrelated Korean donors was performed using a polymerase chain reaction with sequence-specific primers and an allelespecific restriction enzyme analysis. RESULTS: We found that the gene frequencies of HNA-4a and HNA-5a were 0.99 (107/110) and 0.96 (3/110), respectively, among the Korean population. But only the ones of the latter was significantly higher (P<0.01) than in the one of Caucasians. CONCLUSIONS: The gene frequencies of HNA-4a and HNA-5a were determined. We also identified an individual who was the HNA-5a-negative homozygote for the granulocyte panel that could be used for anti-HNA-5a antibody identification.
