Kinetic Analysis of Na-K-ATPase Activity of Rabbit Cerebral Cortex by DDVP.
- Author:
Byoung Jo JANG
1
;
Young Woo LEE
Author Information
1. Department of Neurosurgery, Busan National University, Busan, Korea.
- Publication Type:Original Article
- MeSH:
Cerebral Cortex*;
Dichlorvos*;
Hydrogen-Ion Concentration;
Kinetics
- From:Journal of Korean Neurosurgical Society
1982;11(1):17-22
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The kinetics of inhibition of Na-K-ATPase activity by o, 0-dimethyl o-(2,2-dichlorovinyl) phosphate(DDVP) was investigated with homogenerate of rabbit cerebral cortex. The results were summarized as the follows : 1. Inhibition of Na-K-ATPase activity by DDVP showed a dose-dependent manner with an estimated I5.0 of 3.5x10(-4) M. 2. Altered pH and activity curves for Na-K-ATPase activity demonstrated comparable inhibition by DDVP in buffered acidic, neutral and alkaline pH renges. 3. Kinetic studies of cationic-substrate activation of Na-K-ATPase showed noncompetitive with respect to substrate and K+ and a mixed type inhibition with respect to Na+. These results suggest that DDVP appeared to exert its effects on Na-K-ATPase activity by interfering with the formation of Na-dependent phosphoenzyme.
