Clinical Significance of Fecal Lactoferrin and Multiplex Polymerase Chain Reaction in Patients with Acute Diarrhea.
- Author:
Hae Mi LEE
1
;
Seungok LEE
;
Bo In LEE
;
Dong Wook JEKARL
;
Joo Yong SONG
;
Hye Jung CHOI
;
Bong Koo KANG
;
Eun Joo IM
;
Joon Sung KIM
;
Jong In KIM
;
Byung Wook KIM
;
Hwang CHOI
Author Information
- Publication Type:Original Article ; Evaluation Studies ; Research Support, Non-U.S. Gov't
- Keywords: Acute diarrhea; Fecal lactoferrin; Fecal leukocytes; Multiplex polymerase chain reaction
- MeSH: Adult; Biomarkers/analysis; Dehydration/enzymology/microbiology; Diarrhea/complications/*enzymology/microbiology; Feces/*enzymology/microbiology; Female; Humans; Lactoferrin/*analysis; Male; Multiplex Polymerase Chain Reaction/*statistics & numerical data; Prospective Studies
- From:Gut and Liver 2015;9(5):636-640
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND/AIMS: The diagnostic yield of fecal leukocyte and stool cultures is unsatisfactory in patients with acute diarrhea. This study was performed to evaluate the clinical significance of the fecal lactoferrin test and fecal multiplex polymerase chain reaction (PCR) in patients with acute diarrhea. METHODS: Clinical parameters and laboratory findings, including fecal leukocytes, fecal lactoferrin, stool cultures and stool multiplex PCR for bacteria and viruses, were evaluated prospectively for patients who were hospitalized due to acute diarrhea. RESULTS: A total of 54 patients were included (male, 23; median age, 42.5 years). Fecal leukocytes and fecal lactoferrin were positive in 33 (61.1%) and 14 (25.4%) patients, respectively. Among the 31 patients who were available for fecal pathogen evaluation, fecal multiplex PCR detected bacterial pathogens in 21 patients, whereas conventional stool cultures were positive in only one patient (67.7% vs 3.2%, p=0.000). Positive fecal lactoferrin was associated with presence of moderate to severe dehydration and detection of bacterial pathogens by multiplex PCR (21.4% vs 2.5%, p=0.049; 100% vs 56.5%, p=0.032, respectively). CONCLUSIONS: Fecal lactoferrin is a useful marker for more severe dehydration and bacterial etiology in patients with acute diarrhea. Fecal multiplex PCR can detect more causative organisms than conventional stool cultures in patients with acute diarrhea.
