Expression of p53 Protein and PCNA in Brain Tumors.
- Author:
Hong Soo KIM
1
;
Yeong Hwan AHN
;
Eon Sub PARK
;
Jin Ho MOK
;
Kwan PARK
;
Young Baeg KIM
;
Byung Kook MIN
;
Sung Nam HWANG
;
Jong Sik SUK
;
Duck Young CHOI
Author Information
1. Department of Neurosurgery, College of Medicine, Chung-Ang University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Brain tumor;
P53 protein;
PCNA;
Immunochemistry;
Labelling index
- MeSH:
Antibodies, Monoclonal;
Astrocytoma;
Brain Neoplasms*;
Brain*;
Glioblastoma;
Immunochemistry;
Immunohistochemistry;
Meningioma;
Oligodendroglioma;
Proliferating Cell Nuclear Antigen*
- From:Journal of Korean Neurosurgical Society
1995;24(2):189-194
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Formalin-fixed, paraffin-embedded samples of 32 brain tumors(8 meningiomas, 10 astrocytomas(low grade 5, anaplastic 5), 8 glioblastoma multiforme, 6 oligodendrogliomas(low grade 2, anaplastic 3) were investigated by immunocytochemistry with monoclonal antibodies against p53 protein and proliferating cell nuclear antigen(PCNA). Expression of p53 protein was detected in 1 of 5 cases(20%) of low grade astrocytomas, 2 of 5 cases(40%) of anaplastic astrocytoma, 4 of 8 cases(50%) of glioblastoma multiforme and 4 of 4 cases(100%) of malignant oligodendroglioma. All low grade oligodendrogliomas, meningiomas fail to express p53. Expression of PCNA was detected in 3 of 8 cases of meningioma with very low labeling index(0-0.3), 2 of 5 cases of low grade astrocytoma with low labeling index(0-11.3), 3 of 5 cases of anaplastic astrocytoma with middle labeling index(0-28.2), 6 of 8 cases of glioblastoma multiforme and 4 of 4 cases of malignant oligodendroglioma with high labelling index(0-92.3, 7.6-48.1). Expression of PCNA was not detected in low grade oligodendrogliomas. Tumor group with high expression of p53 protein showed increment of PCNA expression. A strong positive correlation between tumor grade and extent of p53 protein and PCNA expression was found(p<0.002) .
